Mouse IgG1 PE-Cy™7
Clone X40 (ASR)
- Concentration 50 µg/mL
- Vol. Per Test 5 µL
- Isotype Mouse BALB/c IgG1, κ
Flow cytometry, Isotype control (Routinely Tested)
- Immunogen Keyhole limpet hemocyanin (KLH)
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status ASR
Regulatory Status Legend
The Mouse IgG1 antibody, clone X40, is derived from the hybridization of Sp2/0-Ag 14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.
The antibodies react specifically with keyhole limpet hemocyanin (KLH), an antigen not expressed on human cells or human cell lines.
- Format PE-Cy™7
- Excitation Source Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max 496 nm
- Emission Max 785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
Preparation and Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.