CD41a PerCP-Cy™5.5

BD™ CD41a PerCP-Cy™5.5

Name: CD41a PerCP-Cy™5.5
Brand: BD™
SKU: 340930

Clone HIP8

(ASR)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: ITGA2B; Integrin alpha-2b (αIIb); Platelet glycoprotein IIb (GPIIb)

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Purified platelet membrane glycoproteins

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: IV P38

Entrez Gene ID: 3674

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340930 Rev. 1

Antibody Details

HIP8

The CD41a antibody, clone HIP8, is derived from the hybridization of P3X63 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with purified platelet membrane glycoproteins.

The CD41a antibody recognizes the calcium-dependent gpIIb/IIIa complex, with a molecular weight of 140 kilodaltons (kDa).

340930 Rev. 1

Format Details

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

340930 Rev.1

Citations & References

Development References (6)

Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Feng R, Shimazaki C, Inaba T, et al. CD34+/CD41a+ cells best predict platelet recovery after autologous peripheral blood stem cell transplantation. Bone Marrow Transplant. 1998; 21:1217-1222. (Biology).
Jennings LK, Ashmun RA, Wang WC, Docter ME. Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann’s thrombasthenia in whole blood by ﬂow cytometry. Blood. 1986; 68:173-179. (Biology).
de Haas M, von dem Borne A. CD41/CD61 Workshop Panel report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:643-645.
von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.

View All (6)

340930 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.