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      CD28 PerCP-Cy™5.5
      Product Details
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      BD™
      CD28 antigen; T44; Tp44; TP44; Leu28
      Human
      Mouse BALB/c IgG1, κ
      HPB-ALL T cell line
      Flow cytometry
      6 μg/mL
      20 μL
      7099,940
      Phosphate buffered saline with gelatin and sodium azide.
      ASR


      Preparation And Storage

      Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      655734 Rev. 1
      Antibody Details
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      L293

      The CD28 antibody, clone L293, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line.

      The CD28 antigen, a disulfide-linked homodimeric glycoprotein, Mr 44 kilodaltons (kd), is a cell-adhesion molecule (CAM) and functions as the ligand for CD80 (B7-1) and CD86 (B7-2) antigens, which are present on activated B lymphocytes, monocytes, and dendritic cells. Interaction of the CD28 antigen with CD80 or CD86 antigens, or both, co-stimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated proliferation and cytotoxicity.

      655734 Rev. 1
      Format Details
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      PerCP-Cy5.5
      PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PerCP-Cy5.5
      Blue 488 nm
      482 nm
      676 nm
      655734 Rev.1
      Citations & References
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      Development References (17)

      1. Azuma M, Cayabyab M, Buck D, Phillips JH, Lanier LL. CD28 interaction with B7 costimulates primary allogeneic proliferative responses and cytotoxicity mediated by small, resting T lymphocytes.. J Exp Med. 1992; 175(2):353-60. (Biology). View Reference
      2. Azuma M, Phillips JH, Lanier LL. CD28- T lymphocytes. Antigenic and functional properties.. J Immunol. 1993; 150(4):1147-59. (Biology). View Reference
      3. Caux C, Vanbervliet B, Massacrier C, et al. B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994; 180:1841-1847. (Biology).
      4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      5. Fleischer J, Soeth E, Reiling N, Grage-Griebenow E, Flad HD, Ernst M. Differential expression and function of CD80 (B7-1) and CD86 (B7-2) on human peripheral blood monocytes. Immunology. 1996; 89:592-598. (Biology).
      6. Freeman GJ, Freedman AS, Segil JM, Lee G, Whitman JF, Nadler LM. B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells. J Immunol. 1989; 143:2714-2722. (Biology).
      7. Hara R, Fu SM, Hansen JA. Human T-cell activation, II: A new activation pathway used by a major T-cell population via a disulfide-bonded dimer of a 44-kilodalton polypeptide (9-3 antigen). J Exp Med. 1985; 161:1513-1524. (Biology).
      8. June CH, Ledbetter JA, Linsley PS, Thompson CB. Role of the CD28 receptor in T-cell activation. Immunol Today. 1990; 11:211-216. (Biology).
      9. Lanier LL, O'Fallon S, Somoza C, et al. CD80 (B7) and CD86 (B70) provide similar co-stimulatory signals for T cell proliferation, cytokine production, and generation of CTL. J Immunol. 1995; 154:97-105. (Biology).
      10. Lenschow DJ, Walunas TL, Bluestone JA. CD28/B7 system of T cell costimulation. Annu Rev Immunol. 1996; 14:233-258. (Biology). View Reference
      11. Levine BL, Ueda Y, Craighead N, Huang ML, June CH. CD28 ligands CD80 (B7-1) and CD86 (B7-2) induce long-term autocrine growth of CD4+ T cells and induce similar patterns of cytokine secretion in vitro. Int Immunol. 1995; 7:891-904. (Biology).
      12. Linsley PS, Greene JL, Brady W, Bajorath J, Ledbetter JA, Peach R. Human B7-1 (CD80) and B7-2 (CD86) bind with similar avidities but distinct kinetics to CD28 and CTLA-4 receptors. Immunity. 1994; 1:793-801. (Biology).
      13. Morishita Y, Sao H, Hansen JA, Martin PJ. A distinct subset of human CD4+ cells with a limited alloreactive T cell receptor repertoire. Immunol Today. 1989; 143:2783-2789. (Biology).
      14. Nunes J, Klasen S, Ragueneau M, et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993; 5(3):311-315. (Biology). View Reference
      15. Protection of Laboratory Workers from Occupationally Acquired Infections. Approved Guideline. 2005; M29-A3. (Biology).
      16. Rotteveel FT, Kokkelink I, van Lier RA, et al. Clonal analysis of functionally distinct human CD4+ T cell subsets. J Exp Med. 1988; 168(5):1659-1673. (Biology). View Reference
      17. Young JW, Koulova L, Soergel SA, Clark EA, Steinman RM, Dupont B. The B7/BB1 antigen provides one of several co-stimulatory signals for the activation of CD4+ T lymphocytes by human blood dendritic cells in vitro. J Clin Invest. 1992; 90:229-237. (Biology).
      View All (17) View Less
      655734 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Analyte Specific Reagent. Analytical and performance characteristics are not established.

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