Anti-Terminal-Deoxynucleotidyl Transferase (TdT) APC
Clone E17-1519 (ASR)
- Concentration 25 μg/mL
- Vol. Per Test 5 μL
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Intracellular Staining, Flow cytometry (Routinely Tested)
- Immunogen Purified Human TdT
- Storage Buffer Phosphate buffered saline with gelatin and 0.1% sodium azide.
- Regulatory Status ASR
Regulatory Status Legend
Anti-TdT, clone E17-1519, is generated from the fusion of FO myeloma cells with spleen cells from BALB/c mice immunized with purified TdT enzyme.
Anti–terminal-deoxynucleotidyl transferase (TdT) recognizes a 60-kilodalton (kd) polymerase, a nuclear enzyme that catalyzes the template-independent addition of nucleotides to single-stranded DNA primers. It has been reported that TdT is involved in the regulation or translocation or both of DNA and gene rearrangement during normal T -and B-cell development.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Preparation and Storage
Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.