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      Purified NA/LE Rat Anti-Mouse CD14
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat DA, also known as DA/HA IgG2b, κ
      Mouse monocyte/macrophage cell line
      Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
      1.0 mg/ml
      AB_396926
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      557896 Rev. 3
      Antibody Details
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      4C1/CD14

      The 4C1/CD14 antibody reacts with CD14, a ~55-kDa glycosyl phosphatidyl inositol (GPI)-linked cell-surface glycoprotein.  Flow cytometric analysis demonstrates that 4C1/CD14 antibody stains CD14 transfectants, the monocyte/macrophage cell line RAW 264.7 peritoneal exudate neutrophils and macrophages, and cultured bone marrow-derived macrophages, but not undifferentiated bone marrow cells, splenocytes, or peripheral blood leukocytes. This staining pattern is similar to that of the alternate anti-mouse CD14 mAb rmC5-3, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils.  Therefore, data suggest that CD14 expression by leukocyte populations may differ in mice and humans. CD14 is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein). After binding to LPS-LPB, CD14 associates with integral membrane proteins (such as TLR4-MD-2) which mediate signal transduction. It is involved in the development of endotoxic shock, innate immunity, inflammatory responses, and LPS-stimulated bone resorption. Unlike mAb rmC5-3, the 4C1/CD14 antibody blocks the binding of LPS to CD14.

      557896 Rev. 3
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      557896 Rev.3
      Citations & References
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      Development References (12)

      1. Adachi Y, Satokawa C, Saeki M, et al. Inhibition by a CD14 monoclonal antibody of lipopolysaccharide binding to murine macrophages. J Endotoxin Res. 1999; 5(3):139-146. (Immunogen: Blocking, Immunoprecipitation).
      2. Akashi S, Saitoh S, Wakabayashi Y, et al. Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14. J Exp Med. 2003; 198(7):1035-1042. (Clone-specific: Immunoprecipitation). View Reference
      3. Haziot A, Ferrero E, Kontgen F, et al. Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice. Immunity. 1996; 4(4):407-414. (Biology). View Reference
      4. Heumann D, Adachi Y, Le Roy D, et al. Role of plasma, lipopolysaccharide-binding protein, and CD14 in response of mouse peritoneal exudate macrophages to endotoxin. Infect Immun. 2001; 69(1):378-385. (Clone-specific: Blocking). View Reference
      5. Le Roy D, Di Padova F, Adachi Y, Glauser MP, Calandra T, Heumann D. Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria. J Immunol. 2001; 167(5):2759-2765. (Clone-specific: Blocking). View Reference
      6. Matsuura K, Ishida T, Setoguchi M, Higuchi Y, Akizuki S, Yamamoto S. Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide. J Exp Med. 1994; 179(5):1671-1676. (Clone-specific: Flow cytometry, Western blot). View Reference
      7. Miyata Y, Takeda H, Kitano S, Hanazawa S. Porphyromonas gingivalis lipopolysaccharide-stimulated bone resorption via CD14 is inhibited by broad-spectrum antibiotics. Infect Immun. 1997; 65(9):3513-3519. (Biology). View Reference
      8. Nagaoka I, Hirota S, Niyonsaba F, et al. Cathelicidin family of antibacterial peptides CAP18 and CAP11 inhibit the expression of TNF-alpha by blocking the binding of LPS to CD14(+) cells. J Immunol. 2001; 167(6):3329-3338. (Biology). View Reference
      9. Tasaka S, Ishizaka A, Yamada W, et al. Effect of CD14 blockade on endotoxin-induced acute lung injury in mice. Am J Respir Cell Mol Biol. 2003; 29(2):252-258. (Clone-specific: Blocking). View Reference
      10. Triantafilou M, Triantafilou K. Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster. Trends Immunol. 2002; 23(6):301-304. (Biology). View Reference
      11. Yang S, Sugawara S, Monodane T, et al. Micrococcus luteus teichuronic acids activate human and murine monocytic cells in a CD14- and toll-like receptor 4-dependent manner. Infect Immun. 2001; 69(4):2025-2030. (Clone-specific: Blocking). View Reference
      12. Ziegler-Heitbrock HW. Heterogeneity of human blood monocytes: the CD14+ CD16+ subpopulation. Immunol Today. 1996; 17(9):424-428. (Biology). View Reference
      View All (12) View Less
      557896 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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