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      Flex Single-Cell Multiplexing Kit B, Flex Sample Tag 7-12

      BD® Flex Single-Cell Multiplexing Kit B, Flex Sample Tag 7-12

      Product Details
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      BD®
      Single cell multiomics experiments on the BD Rhapsody™ platform (Tested During Development)
      RUO


      Description

         The BD® Flex Sample Multiplexing Kit B Tag 7-12 contains the necessary reagents to support flexible labelling up to 6 single-cell samples (Flex SMK Sample Tags 7-12). If combined with Flex SMK A, C, and D Kits, up to 24 samples can be labelled and pooled before single-cell capture with the BD Rhapsody™ Single-Cell Analysis system and BD Rhapsody™ Express Single-Cell Analysis system.

         The E31-1459 monoclonal  antibody specifically recognizes R-Phycoerythrin (PE), a commonly used fluorochrome for flow cytometry. The E31-1459 hybridoma was generated using purified  R-Phycoerythrin as the immunogen.  The E31-1459 antibody allows for the use of any R-PE conjugated primary antibody to stain sample cells of interest with a two-step staining approach, regardless of cell type or species origin. The binding of the E31-1459 antibody to PE does not quench the fluorescence of the PE molecule. E31-1459 antibody also binds to R-PE tandem fluorophores (eg, PE-Cy™7). User should be mindful if using a multi-color flow sorting panel with PE or PE-tandem conjugates prior to single cell multiomic analysis.

      Recommended Assay Procedures

      Please refer to protocols Single Cell Labeling with the BD® Flex Single-Cell Multiplexing Kits (Doc ID: 23-24311) and Single Cell Labeling with the BD® Flex Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (Doc ID:23-24312). Before using reagents, see the BD Rhapsody™ Single-Cell Analysis System Instrument User Guide (Doc ID: 214062) or the BD Rhapsody™ Express Single-Cell Analysis System Instrument User Guide (Doc ID: 214063).

      Use standard laboratory safety protocols. Read and understand the safety data sheets (SDSs) before handling chemicals. To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at scomix@bdscomix.bd.com.

      Warning: All biological specimens and materials coming in contact with them are considered biohazardous. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.

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      Product Notices

      1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. For U.S. patents that may apply, see bd.com/patents.
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      633850 Rev. 2
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      Flex Single-Cell Multiplexing Kit B Sample Tag 7 2 Tests (1 ea) 51-9021467 N/A
      Flex Single-Cell Multiplexing Kit B Sample Tag 8 2 Tests (1 ea) 51-9021499 N/A
      Flex Single-Cell Multiplexing Kit B Sample Tag 9 2 Tests (1 ea) 51-9021500 N/A
      Flex Single-Cell Multiplexing Kit B Sample Tag 10 2 Tests (1 ea) 51-9021501 N/A
      Flex Single-Cell Multiplexing Kit B Sample Tag 11 2 Tests (1 ea) 51-9021502 N/A
      Flex Single-Cell Multiplexing Kit B Sample Tag 12 2 Tests (1 ea) 51-9021503 N/A
      633850 Rev. 2
      Citations & References
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      Development References (3)

      1. Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level.. STAR Protoc. 2020; 1(2):100092. (Methodology). View Reference
      2. Mavrangelos C, Swart B, Nobbs S, Nicholson IC, Macardle PJ, Zola H. Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagents.. J Immunol Methods. 2004; 289(1-2):169-78. (Clone-specific: Flow cytometry). View Reference
      3. Shum EY, Walczak EM, Chang C, Christina Fan H. Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System.. Adv Exp Med Biol. 2019; 1129:63-79. (Methodology). View Reference
      633850 Rev. 2

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.