Preparation And Storage
Recommended Assay Procedures
Applications include immunohistochemistry of paraformaldehye-fixed, frozen (1 µg/ml) or vibratome tissue sections (0.1-1.0 µg/ml), western blot, and immunoelectron microscopy.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
The catecholamine neurotranmitters-dopamine, noradrenaline, and adrenaline-are involved in multiple physiological processes and neurological disorders. These three transmitters are synthesized from the amino acid tyrosine in a common pathway that uses five enzymes: tyrosine hydroxylase, aromatic amino acid decarboxylase, dopamine β hydroxylase, pteridine reductase, phenylethanolamine-N-methyl transferase. Tyrosine hydroxylase is the rate-limiting enzyme in catecholamine synthesis. It converts tyrosine to L-dihydroxyphenylalanine (L-DOPA), which is then further converted to give rise to dopamine, noradrenaline, and adrenaline. Antibodies against these biosynthetic enzymes have been widely used in the immunohistochemical analysis of catecholaminergic neurons. TOH A1 specifically reacts with rat tyrosine hydroxylase and can be used to identify tyrosine hydroxylase containing catecholaminergic neurons in rat brain.
Development References (2)
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Schwartz HH. Chemical messengers: Small molecules and peptides. In: Kandel ER, Schwartz JH, Jessel TM, ed. Principles of Neural Science. New York: Elseviers; 1991:213-224.
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Semenenko FM, Cuello AC, Goldstein M, Lee KY, Sidebottom E. A monoclonal antibody against tyrosine hydroxylase: application in light and electron microscopy. J Histochem Cytochem. 1986; 34(6):817-821. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
