Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The Sos (son of sevenless) gene was originally identified in Drosophila, and two mammalian homologues (mSos1 and mSos2) were isolated from a mouse cDNA library. These two cDNAs predict proteins that are approximately 70% identical in their amino acid residues. Both mSos1 and mSos2 are expressed in a wide number of mouse embryonic and adult tissues as well as in several cell lines. The human homologues, hSos1 and hSos2 have also been isolated and show a very high degree of amino acid identity to the mouse genes (98% for Sos1). Sos1 has a predicted molecular weight of 150kDa, but the apparent molecular weight is closer to 170 kDa, presumably due to a high proline content. The mammalian Sos1 protein has a highly specific guanine nucleotide exchange activity toward p21ras . In EGF-stimulated cells, Sos1 interacts with the SH3 domains of GRB2, and GRB2 binds via its SH2 domain to tyrosine 1068 of the activated EGF receptor. Thus, GRB2 recruits Sos1 to the plasma membrane and enables it to activate the Ras signaling pathway by enhancing GTP loading on p21ras .
Development References (5)
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Buday L, Downward J. Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor. Cell. 1993; 73(3):611-620. (Biology). View Reference
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Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA. Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Nature. 1993; 363(6424):45-51. (Biology). View Reference
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Furuta S, Miura K, Copeland T, Shang WH, Oshima A, Kamata T. Light Chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling. Oncogene. 2002; 21(46):7060-7066. (Clone-specific: Immunofluorescence). View Reference
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Kardinal C, Konkol B, Lin H, et al. Chronic myelogenous leukemia blast cell proliferation is inhibited by peptides that disrupt Grb2-SoS complexes. Blood. 2001; 98(6):1773-1781. (Clone-specific: Western blot). View Reference
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Salojin KV, Zhang J, Meagher C, Delovitch TL. ZAP-70 is essential for the T cell antigen receptor-induced plasma membrane targeting of SOS and Vav in T cells. J Biol Chem. 2000; 275(8):5966-5975. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
