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Purified Mouse Anti-Human HS1
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human HS1 aa. 17-190
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
75 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610541 Rev. 1
Antibody Details
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HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610541 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610541 Rev.1
Citations & References
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Development References (3)

  1. Fukuda T, Kitamura D, Taniuchi I. Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate. Proc Natl Acad Sci U S A. 1995; 92(16):7302-7306. (Biology). View Reference
  2. Takemoto Y, Furuta M, Li XK, Strong-Sparks WJ, Hashimoto Y. LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck. EMBO J. 1995; 14(14):3403-3414. (Biology). View Reference
  3. Yamanashi Y, Okada M, Semba T. Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling. Proc Natl Acad Sci U S A. 1993; 90(8):3631-3635. (Biology). View Reference
610541 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.