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Purified Mouse Anti-Gelsolin
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Rabbit (Tested in Development)
Mouse IgG2a
Human Gelsolin aa. 592-768
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
93 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610412 Rev. 1
Antibody Details
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Gelsolin was identified as a result of its ability to sever actin filaments in a Ca2+-dependent manner. The gene for gelsolin encodes an 83 kDa protein that migrates as a 93 kDa polypeptide in SDS-gels. The N-terminal domain contains the calcium-independent actin-severin site, whereas the calcium-dependent site is located in the C-terminal portion of the protein. It exhibits significant homology with villin, another calcium-regulated actin filament severing protein. Gelsolin can be found intracellularly, as well as in a secreted form. However, both forms are encoded by the same gene.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610412 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610412 Rev.1
Citations & References
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Development References (5)

  1. Arnt CR, Chiorean MV, Heldebrant MP, Gores GJ, Kaufmann SH. Synthetic Smac/DIABLO peptides enhance the effects of chemotherapeutic agents by binding XIAP and cIAP1 in situ. J Biol Chem. 2002; 277(46):44236-44243. (Clone-specific: Western blot). View Reference
  2. De Botton S, Sabri S, Daugas E, et al. Platelet formation is the consequence of caspase activation within megakaryocytes. Blood. 2002; 100(4):1310-1317. (Clone-specific: Western blot). View Reference
  3. Kwiatkowski DJ, Stossel TP, Orkin SH, Mole JE, Colten HR, Yin HL. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature. 1986; 323(6087):455-458. (Biology). View Reference
  4. Slee EA, Adrain C, Martin SJ. Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem. 2001; 276(10):7320-7326. (Clone-specific: Western blot). View Reference
  5. Wang Q, Xie Y, Du QS, et al. Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin. J Cell Biol. 2003; 160(4):565-575. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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610412 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.