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Western blot analysis of Gαt on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Gαt antibody.
Immunofluorescence staining of rat neurons.
BD Transduction Laboratories™ Purified Mouse Anti-Gαt
BD Transduction Laboratories™ Purified Mouse Anti-Gαt
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
The GTP binding regulatory proteins (G proteins) consist of three subunits: α, β, and γ. These heterotrimeric proteins function at membranes to relay signals from cell surface receptors to intracellular effectors. The α subunit is unique for each G protein and contains the site of GTP binding and hydrolysis, as well as sites for receptor and effector interactions. The βγ subunit complex interacts directly with receptors and the α subunit. The Gα protein transducin (Gαt) contains 350 amino acids and has been extensively studied as a model for G protein function. Gαt requires GTP in order to bind to its effectors. In the process of effector-Gαt binding, GTP is hydrolyzed and the βγ subunits are displaced. The free Gαt-GDP then reassociates with the βγ subunits and re-loads GTP to repeat the cycle.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (1)
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Skiba NP, Bae H, Hamm HE. Mapping of effector binding sites of transducin alpha-subunit using G alpha t/G alpha i1 chimeras. J Biol Chem. 1996; 271(1):413-424. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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