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Western blot analysis of panMunc13 on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of panMunc13.
BD Transduction Laboratories™ Purified Mouse Anit-panMunc13
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Release of neurotransmitters at the synaptic junction is a primary mechanism of neuronal communication and is regulated by the synaptic vesicle (SV) cycle. Several components of the SV cycle were initially characterized in C. elegans. One of those, unc-13, is involved in normal presynaptic function. Mammalian homologues of unc-13 are Munc13-1, 13-2, and 13-3. These neuron-specific proteins lack N-terminal homology, but are highly conserved in their C-terminal regions. Here they contain a phorbal ester binding C1-domain and two C2-domains with homology to the PKC Ca2+/phospholipid-binding domain. The most abundant isoform, Munc 13-1, is a presynaptic receptor with high affinities for phorbol ester and DAG. In response to phorbol ester, Munc13-1 associates with the plasma membrane and acts as a stimulation dependent enhancer of neurotransmitter release. In addition, Munc13-1 interacts with components of the SV cycle (syntaxin, SNAP25, synaptobrevin, Doc2) and with a brain specific isoform of β-spectrin, a protein that interacts with the actin cytoskeleton. Thus, it is thought that Munc13 proteins function in signaling pathways that regulate the neuronal exocytic machinery.
Development References (4)
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Betz A, Ashery U, Rickmann M. Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release. Neuron. 1998; 21(1):123-136. (Biology). View Reference
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Brose N, Hofmann K, Hata Y, Sudhof TC. Mammalian homologues of Caenorhabditis elegans unc-13 gene define novel family of C2-domain proteins. J Biol Chem. 1995; 270(42):25273-25280. (Biology). View Reference
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Orita S, Naito A, Sakaguchi G. Physical and functional interactions of Doc2 and Munc13 in Ca2+-dependent exocytotic machinery. J Biol Chem. 1997; 272(26):16. (Biology). View Reference
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Sakaguchi G, Orita S, Naito A. A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13. Biochem Biophys Res Commun. 1998; 248(3):846-851. (Biology). View Reference
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