Preparation And Storage
Recommended Assay Procedures
ELISA Capture: The purified BVD13-3A5 antibody (Cat. No. 551342) is useful as a capture antibody for a sandwich ELISA for measuring human G-CSF protein levels. Purified BVD13-3A5 antibody can be paired with the biotinylated BVD11-37G10 antibody (Cat. No. 554670) as the detecting antibody, with recombinant human G-CSF as the standard. Purified BVD13-3A5 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human BVD13-3A5 ranging from ~2,000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, and go to the protocols section or refer to the chapter on ELISA in the Immune Function Handbook both of which can be found at www.bdbiosciences.com.
WB: The BVD13-3A5 antibody (Cat. No. 551342) has been found useful for Western blotting. A concentration of 1-5 µg/ml has been found to enable visualization of ≤ 100 ng/lane of recombinant human G-CSF, under reducing conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The BVD13-3A5 antibody reacts with human granulocyte-colony stimulating factor (G-CSF). The immunogen used to generate the hybridoma was E. coli-expressed human G-CSF. This is a neutralizing antibody.
Development References (2)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.