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      RY586 Rat Anti-Mouse CD104
      Product Details
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      BD OptiBuild™
      β4 integrin; Integrin β4 chain; Integrin beta-4; Itgb4; ITB4
      Mouse (Tested in Development)
      Rat F344, also known as Fischer, CDF IgG2a, κ
      Tumor-associated antigen TSP-180 from a BALB/c mouse lung carcinoma
      Flow cytometry (Qualified)
      0.2 mg/ml
      192897
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      753559 Rev. 1
      Antibody Details
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      346-11A

      The 346-11A monoclonal antibody specifically recognizes an epitope at the beginning of the cysteine-rich repeat region of the 200-kDa integrin β4 chain (CD104), which is found on the cell surface as a heterodimeric complex with the integrin α6 chain (CD49f). The α6β4 (CD49f/CD104) complex binds to laminins and is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia, and is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes. It has also been identified on a number of tumor tissues and participates in tumor progression events. Localization of both human and mouse epidermal α6β4 integrin to hemidesmosomes suggests that this heterodimer plays a role in epidermal adhesion to the basement membrane.

      753559 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753559 Rev.1
      Citations & References
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      Development References (8)

      1. Giancotti FG. Signal transduction by the alpha 6 beta 4 integrin: charting the path between laminin binding and nuclear ev. J Cell Sci. 1996; 109(6):1165-1172. (Biology). View Reference
      2. Kennel SJ, Foote LJ, Falcioni R, et al. Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily. J Biol Chem. 1989; 264(26):15515-15521. (Clone-specific: Immunoprecipitation, Western blot). View Reference
      3. Kennel SJ, Foote LJ, Flynn KM. Tumor antigen on benign adenomas and on murine lung carcinomas quantitated by a two-site monoclonal antibody assay. Cancer Res. 1986; 46(2):707-712. (Immunogen: Blocking, Immunoprecipitation, Radioimmunoassay). View Reference
      4. Morena A, Riccioni S, Marchetti A, et al. Expression of the beta 4 integrin subunit induces monocytic differentiation of 32D/v-Abl cells. Blood. 2002; 100(1):96-106. (Biology). View Reference
      5. Salmivirta K, Gullberg D, Hirsch E, Altruda F, Ekblom P. Integrin subunit expression associated with epithelial-mesenchymal interactions during murine tooth development. Dev Dyn. 1996; 205(2):104-113. (Clone-specific: Immunohistochemistry). View Reference
      6. Sonnenberg A, Calafat J, Janssen H, et al. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.. J Cell Biol. 1991; 113(4):907-17. (Clone-specific: Immunohistochemistry). View Reference
      7. Sonnenberg A, Linders CJ, Daams JH, Kennel SJ. The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J Cell Sci. 1990; 96(2):207-217. (Clone-specific: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
      8. van der Neut R, Krimpenfort P, Calafat J, Niessen CM, Sonnenberg A. Epithelial detachment due to absence of hemidesmosomes in integrin beta 4 null mice. Nat Genet. 1996; 13(3):366-369. (Biology). View Reference
      View All (8) View Less
      753559 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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