Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The BZ2E3 monoclonal antibody specifically binds to CCRL2 (C-C chemokine receptor-like 2) which is also known as Lipopolysaccharide-inducible C-C chemokine receptor (L-CCR/Lccr). CCRL2 is a chemokine receptor-like, 7-transmembrane protein that can bind CCL19 or the nonchemokine chemoattractant, chemerin/RARRES2. CCRL2 is expressed by a variety of cell types including mast cells, macrophages and some endothelial cells. CCRL2 appears to play various roles in regulating chemokine activities, e.g., by binding and internalizing excess local levels of the CCL19/MIP-3β chemokine. Alternatively, endothelial cell-expressed CCRL2 can bind and present the chemerin/RARRES2 chemoattractant to lymphoid cells that express the functional signaling receptor, CMKLR1, and promote their adhesion.
Development References (4)
-
Monnier J, Lewen S, O'Hara E, et al. Expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells. J Immunol. 2012; 189(2):956-967. (Clone-specific: Flow cytometry). View Reference
-
Shimada T, Matsumoto M, Tatsumi Y, Kanamaru A, Akira S. A novel lipopolysaccharide inducible C-C chemokine receptor related gene in murine macrophages. FEBS Lett. 1998; 425(3):490-494. (Biology). View Reference
-
Yoshimura T, Oppenheim JJ. Chemokine-like receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2); two multifunctional receptors with unusual properties. Exp Cell Res. 2011; 317(5):674-684. (Biology). View Reference
-
Zabel BA, Nakae S, Zúñiga L, et al. Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J Exp Med. 2008; 205(10):2207-2220. (Immunogen: Blocking, ELISA, Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
