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RY586 Mouse Anti-Rat CD6
Product Details
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BD OptiBuild™
OX-52 Antigen; Pan-T Cells; Cd6; Tp120
Rat (Tested in Development)
Mouse BALB/c IgG2a, κ
PVG-RT1c rat thoracic-duct leukocytes and splenocytes
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. CF™ is a trademark of Biotium, Inc.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
753440 Rev. 1
Antibody Details
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OX-52

The OX-52 monoclonal antibody specifically recognizes CD6, a 95-120–kDa antigen expressed on T lymphocytes and thymocytes expressing αβ and γδ Tcell receptors. Rat NK cells express low levels of CD6 and are CD8a+, CD8b-, CD5-, and CD3-. In immunoprecipitation experiments, CD6 has been shown to co-precipitate with CD5 and to play a key role in CD5 tyrosine phosphorylation.

753440 Rev. 1
Format Details
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RY586
The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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RY586
Yellow-Green 561 nm
564 nm
586 nm
753440 Rev.1
Citations & References
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View product citations for antibody "753440" on CiteAb

Development References (5)

  1. Brunson ME, Tchervenkov JI, Alexander JW, Cofer BR. Partial T-cell depletion with monoclonal antibody improves the enhancing effect of donor-specific transfusion plus cyclosporine. Transplant Proc. 1991; 23(1):307-308. (Clone-specific: Depletion). View Reference
  2. Castro MA, Nunes RJ, Oliveira MI. OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation. J Leukoc Biol. 2003; 73(1):183-190. (Clone-specific: Immunoprecipitation, Stimulation). View Reference
  3. Lawetzky A, Tiefenthaler G, Kubo R, Hunig T. Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta. Eur J Immunol. 1990; 20(2):343-349. (Clone-specific: Immunoprecipitation). View Reference
  4. Robinson AP, Puklavec M, Mason DW. MRC OX-52: a rat T-cell antigen. Immunology. 1986; 57(4):527-531. (Immunogen: Immunoprecipitation). View Reference
  5. Vaage JT, Dissen E, Ager A, Fossum S, Rolstad B. Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized. Eur J Immunol. 1991; 21(9):2167-2175. (Biology). View Reference
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753440 Rev. 1

 

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.