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      RY586 Mouse Anti-Mouse Ly-49H
      Product Details
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      BD OptiBuild™
      Ly49h; Lymphocyte antigen 49H; Klra8; Cmv1; Cmv-1
      Mouse (Tested in Development)
      Mouse BALB/c IgG1, κ
      Mouse Ly-49A/H Transfected Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      16639
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      753631 Rev. 1
      Antibody Details
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      3D10

      The 3D10 monoclonal antibody specifically binds to mouse Lymphocyte antigen 49H (Ly-49H; also known as Klra8 or Killer cell lectin-like receptor 8). The 3D10 antibody does not crossreact with related molecules such as Ly-49A, C, D or G2. Ly-49H is a type II transmembrane protein and a member of the Ly-49 C-type lectin multigene family of receptors expressed by NK cells. Cell surface Ly-49H is expressed by a subset of NK cells but not by NKT cells. Ly-49H is expressed by C57BL/6 and NWNA but not by BALB/c or DBA/2 mouse NK cells. Cell surface Ly-49H presents as a ~110 kDa disfulfide-linked homodimer and associates with signaling subunits such as DAP10 and DAP12 for optimal transduction of intracellular activation signals. Crosslinking of Ly-49H with the 3D10 antibody reportedly induces NK cell cytotoxicity and cytokine production. Ly-49H recognizes the mouse cytomegalovirus m157 glycoprotein that is expressed by infected cells and is required for protection against cytomegalovirus infection.

      753631 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753631 Rev.1
      Citations & References
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      Development References (5)

      1. Brennan J, Mager D, Jefferies W, Takei F. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J Exp Med. 1994; 180(6):2287-2295. (Biology). View Reference
      2. Brown MG, Dokun AO, Heusel JW, et al. Vital involvement of a natural cell activation receptor in resistance to viral infection. Science. 2001; 292(5518):934-937. (Clone-specific: Activation, Bioassay, Blocking, Cytotoxicity, Flow cytometry). View Reference
      3. Orr MT, Sun JC, Hesslein DG, et al. Ly49H signaling through DAP10 is essential for optimal natural killer cell responses to mouse cytomegalovirus infection. J Exp Med. 2009; 206(4):807-817. (Clone-specific: Blocking, Flow cytometry). View Reference
      4. Silver ET, Elliott JF, Kane KP. Alternatively spliced Ly-49D and H transcripts are found in IL-2-activated NK cells. Immunogenetics. 1996; 44(6):478-482. (Biology). View Reference
      5. Smith HR, Chuang HH, Wang LL, Salcedo M, Heusel JW, Yokoyama WM. Nonstochastic Coexpression of activation receptors on murine Natural Killer cells. J Exp Med. 2000; 191(8):1341-1354. (Immunogen: Activation, Cytotoxicity, Flow cytometry, Immunoprecipitation). View Reference
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      753631 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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