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      RY586 Mouse Anti-Mouse CD229
      Product Details
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      BD OptiBuild™
      Lgp100; Lymphocyte antigen 9; Ly-9; Ly9; SLAM family member 3; SLAMF3; T100
      Mouse (Tested in Development)
      Mouse BALB/c IgG1, κ
      Mouse Ly9 Transfected Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      17085
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      753489 Rev. 1
      Antibody Details
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      Ly9.7.144

      The LY9.7.144 monoclonal antibody specifically recognizes CD229 which is also known as SLAM family member 3 (SLAMF3), Lymphocyte antigen 9 (Ly-9), Lgp100, and T100. CD229 is a ~90-120 kDa type I transmembrane glycoprotein that is encoded by Ly9 (lymphocyte antigen 9) which belongs to the Signaling Lymphocytic Activation Molecule (SLAM) family within the immunoglobulin gene superfamily. The extracellular domain of CD229 contains four extracellular Ig-like domains (2 V-set and 2 C-set) extending in order from the N-terminus as V-C2-V-C2. The intracellular region of CD229 contains two ITSMs (immunoreceptor tyrosine-based switch motifs) and serves as a docking site for SH2-binding phosphatases and adapter molecules including SAP and EAT-2. CD229 is variably expressed on most thymocytes, T cells, NKT cells, B cells, NK cells, hematopoietic stem cells and progenitor cells, and monocytes. CD229 participates in homophilic adhesion interactions between these cells and in the regulation of their activation.

      753489 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753489 Rev.1
      Citations & References
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      Development References (5)

      1. Cuenca M, Punet-Ortiz J, Ruart M, Terhorst C, Engel P. Ly9 (SLAMF3) receptor differentially regulates iNKT cell development and activation in mice. Eur J Immunol. 2018; 48(1):99-105. (Clone-specific: Functional assay, Inhibition, In vivo exacerbation). View Reference
      2. Romero X, Zapater N, Calvo M, et al. CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005; 174(11):7033-7042. (Biology). View Reference
      3. Sandrin MS, Gumley TP, Henning MM, et al. Isolation and characterization of cDNA clones for mouse Ly-9. J Immunol. 1992; 149(5):1636-1641. (Biology). View Reference
      4. Sintes J, Cuenca M, Romero X, et al. Cutting edge: Ly9 (CD229), a SLAM family receptor, negatively regulates the development of thymic innate memory-like CD8+ T and invariant NKT cells. J Immunol. 2013; 190(1):21-26. (Immunogen: Flow cytometry, Fluorescence quantitation, Inhibition, In vivo exacerbation). View Reference
      5. Sintes J, Vidal-Laliena M, Romero X, Tovar V, Engel P. Characterization of mouse CD229 (Ly9), a leukocyte cell surface molecule of the CD150 (SLAM) family. Tissue Antigens. 2007; 70(5):355-362. (Biology). View Reference
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      753489 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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