-
Your selected country is
United States
- Change country/language
-
Training
- Flow Cytometry Basic Training
-
Product-Based Training
- BD FACSDiscover™ S8 Cell Sorter Product Training
- Accuri C6 Plus Product-Based Training
- FACSAria Product Based Training
- FACSCanto Product-Based Training
- FACSLyric Product-Based Training
- FACSMelody Product-Based Training
- FACSymphony Product-Based Training
- HTS Product-Based Training
- LSRFortessa Product-Based Training
- Advanced Training
-
- BD FACSDiscover™ S8 Cell Sorter Product Training
- Accuri C6 Plus Product-Based Training
- FACSAria Product Based Training
- FACSCanto Product-Based Training
- FACSLyric Product-Based Training
- FACSMelody Product-Based Training
- FACSymphony Product-Based Training
- HTS Product-Based Training
- LSRFortessa Product-Based Training
- United States (English)
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 753522) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
BD OptiBuild™ RY586 Mouse Anti-Human TCR Cβ1
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The JOVI.1 monoclonal antibody recognizes an epitope common to a large proportion of human CD4+ or CD8+ T lymphocytes that express the T cell receptor beta chain (TCRβ). The antibody was generated from a mouse immunized with transgenic mouse lymphoid cells that expressed the rearranged human Vβ3-Cβ1 TCR chain derived from the cloned human HA1.7 T helper cell. This antibody reacts with TCR-Cβ1+ T cells and one of several different TCRβ V regions, but not with TCR-Cβ2+ T cells. JOV1.1 antibody reportedly recognized several Cβ1 TCR expressing cell lines or clones including Jurkat, CH7C17, and HA1.7 cells. The JOVI.1 antibody can be used to stimulate proliferative responses by JOVI.1-positive T cells. It can also reportedly be used for immunoprecipitation and to stain JOVI.1+ T cells in frozen tissue sections.
Development References (3)
-
Gil D, Schamel WWA, Montoya M, Sanchez-Madrid F, and Alarcon B. Recruitment of Nck by CD3-epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell. 2002; 109(7):901-912. (Clone-specific: Activation, Functional assay, Immunoprecipitation). View Reference
-
San José E, Alarcón B. Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway.. J Biol Chem. 1999; 274(47):33740-6. (Clone-specific: Immunoprecipitation). View Reference
-
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ. Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992; 11(6):701-713. (Immunogen: Activation, Bioassay, Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Stimulation). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Report a Site Issue
This form is intended to help us improve our website experience. For other support, please visit our Contact Us page.