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      RY586 Mouse Anti-Human SUSD2
      Product Details
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      BD OptiBuild™
      sushi domain-containing protein 2; Sushi domain (SCR repeat) containing; BK65A6.2; testicular tissue protein Li 190
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human retinoblastoma Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      753408 Rev. 1
      Antibody Details
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      W5C5

      The W5C5 monoclonal antibody specifically reacts with SUSD2 (sushi domain containing 2), which is expressed on mesenchymal stem cells (MSC) and on some cancers and neoplastic cell lines.  It is not detected on hematopoietic cells of the peripheral blood and bone marrow. SUSD2 is a type I transmembrane protein that may interact with galectin-1 and the potential cytokine C10orf99, through which it may affect cell growth and immune responsiveness. It has a large extracellular region that contains a sushi domain (a conserved sequence that is often associated with proteins of the complement system). SUSD2 is a marker that is useful for identifying and isolating MSC from bone marrow and endometrial tissue, and those MSC may be induced to differentiate into a wide variety of cell types. Varying levels of SUSD2 expression may correlate with either suppression or enhancement of the growth of different types of neoplastic cells, presumably via a variety of direct and indirect mechanisms.

      753408 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753408 Rev.1
      Citations & References
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      Development References (8)

      1. Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Clone-specific). View Reference
      2. Cheng Y, Wang X, Wang P, et al. SUSD2 is frequently downregulated and functions as a tumor suppressor in RCC and lung cancer.. Tumour Biol. 2016; 37(7):9919-30. (Biology). View Reference
      3. Hultgren EM, Patrick ME, Evans RL, Stoos CT, Egland KA. SUSD2 promotes tumor-associated macrophage recruitment by increasing levels of MCP-1 in breast cancer.. PLoS ONE. 2017; 12(5):e0177089. (Biology). View Reference
      4. Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE. A novel marker of human endometrial mesenchymal stem-like cells.. Cell Transplant. 2012; 21(10):2201-14. (Clone-specific). View Reference
      5. Pan W, Cheng Y, Zhang H, et al. CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.. Sci Rep. 2014; 4:6812. (Biology). View Reference
      6. Sivasubramaniyan K, Harichandan A, Schumann S, et al. Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2.. Stem Cells Dev. 2013; 22(13):1944-54. (Immunogen). View Reference
      7. Ulrich D, Tan KS, Deane J, et al. Mesenchymal stem/stromal cells in post-menopausal endometrium.. Hum Reprod. 2014; 29(9):1895-905. (Clone-specific). View Reference
      8. Watson AP, Evans RL, Egland KA. Multiple functions of sushi domain containing 2 (SUSD2) in breast tumorigenesis.. Mol Cancer Res. 2013; 11(1):74-85. (Biology). View Reference
      View All (8) View Less
      753408 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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