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      RY586 Mouse Anti-Human CD7
      RY586 Mouse Anti-Human CD7
      Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human CD7 antibody (Cat. No. 753349) on human peripheral blood.  Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      RY586 Mouse Anti-Human CD7
      Multiparameter flow cytometric analysis using BD OptiBuild™ RY586 Mouse Anti-Human CD7 antibody (Cat. No. 753349) on human peripheral blood.  Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      Product Details
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      BD OptiBuild™
      GP40; LEU-9; T-cell leukemia antigen; Tp40; TP41
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      P-CLL and Jurkat Cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      IV T163
      924
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      753349 Rev. 2
      Antibody Details
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      M-T701

      The M-T701 monoclonal antibody specifically binds to CD7. CD7 is a 40 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily.  The CD7 antigen is also known as Leu-9, TP41, Tp40, GP40, and T-cell leukemia antigen. It is expressed on thymocytes, T cells, pre-B cells, and NK cells. CD7 is present in reduced density on monocytic cells and cell lines. Functional studies demonstrate that crosslinking of the CD7 can induce transmembrane calcium flux in T cells.

      753349 Rev. 2
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753349 Rev.2
      Citations & References
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      Development References (6)

      1. Lin G-X, Yang X, Hollemweguer E, et al. Cross-reactivity of CD antibodies in eight animal species. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:519-523.
      2. Pace KE, Lee C, Stewart PL, Baum LG. Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1. J Immunol. 1999; 163(7):3801-3811. (Clone-specific: Fluorescence microscopy, Functional assay, Immunofluorescence). View Reference
      3. Rabinowich H, Pricop L, Herberman RB, Whiteside TL. Expression and function of CD7 molecule on human natural killer cells. J Immunol. 1994; 152(2):517-526. (Biology). View Reference
      4. Reiter C. Cluster report: CD7. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:341-342.
      5. Rosenzweig M, Marks DF, DeMaria MA, Connole M, Johnson RP. Identification of primitive hematopoietic progenitor cells in the rhesus macaque. J Med Primatol. 2001; 30(1):36-45. (Clone-specific: Flow cytometry). View Reference
      6. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      View All (6) View Less
      753349 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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