RY586 Mouse Anti-Human CD42a

BD OptiBuild™ RY586 Mouse Anti-Human CD42a

Name: RY586 Mouse Anti-Human CD42a
Brand: BD OptiBuild™
SKU: 753336

Clone ALMA.16

(RUO)

Product Details

Brand: BD OptiBuild™

Alternative Name: GPIX; Glycoprotein IX; GP9; Glycoprotein 9

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: VI P-34

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Researchers should determine the optimal concentration of this reagent for their individual applications.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
CF™ is a trademark of Biotium, Inc.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Human BD Fc Block™ RUO

Size 0.25 mg Cat No. 564220

RY586 Mouse IgG1, κ Isotype Control RUO

Size 50 µg Cat No. 568097

View Details

753336 Rev. 1

Antibody Details

ALMA.16

The ALMA.16 monoclonal antibody specifically recognizes CD42a. CD42a is a 17-22 kDa type I transmembrane glycoprotein that is also known as Platelet glycoprotein IX (GPIX), or Glycoprotein 9 (GP9). CD42a forms a noncovalently linked complex (GPIb/GPIX/GPV) with CD42b, CD42c and CD42d that may serve as a receptor for von Willebrand factor. It is expressed on platelets and megakaryocytes and is absent on the platelets of patients with Bernadr-Soulier Syndrome (BSS). Although the CD42a function is not fully understood, GPIX glycoprotein is important for the assembly and membrane expression of the CD42 complex and for the maintenance of the functional conformation of CD42b (GPIb).

753336 Rev. 1

Format Details

RY586

The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.

Format: RY586

Excitation Source: Yellow-Green 561 nm

Excitation Max: 564 nm

Emission Max: 586 nm

753336 Rev.1

Citations & References

Development References (6)

Azorsa DO, Moog S, Cazenave J-P, Lanza F. CD42a–d Workshop: Analysis of antibodies recognizing the platelet GPIb/IX/V (CD42a,b,c,d) complex. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:651-653.
De Haas M, Von Dem Borne AEGK. CD42a-d Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:648-650.
Fox JE, Aggerbeck LP, Berndt MC. Structure of the glycoprotein Ib.IX complex from platelet membranes. J Biol Chem. 1988; 263(10):4882-4890. (Biology). View Reference
Hickey MJ, Williams SA, Roth GJ. Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures. Proc Natl Acad Sci U S A. 1989; 86(17):6773-6777. (Biology). View Reference
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Schick PK, Walker J. The acylation of megakaryocyte proteins: glycoprotein IX is primarily myristoylated while glycoprotein Ib is palmitoylated. Blood. 1996; 87(4):1377-1384. (Biology). View Reference

View All (6)

753336 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.