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Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Companion Products
The C398.4A monoclonal antibody specifically binds to Inducible Costimulator (ICOS), which is also known as, CD278, Activation-inducible lymphocyte immunomediatory molecule (AILIM), or H4. ICOS is a type I transmembrane glycoprotein that forms a disulfide-linked homodimer and belongs to the CD28 family within the Ig superfamily. ICOS is expressed on either CD4+ or CD8+ single-positive mature thymocytes, T cells, or subsets of Innate Lymphoid Cells (ILC). Its expression is upregulated on activated T lymphocytes. ICOS is a costimulatory receptor that can bind to the B7-H2 ligand (CD275, ICOS-L) that is expressed on B cells, monocytes, macrophages, dendritic cells, and endothelial cells. ICOS plays a critical role in many types of T cell-dependent immunity. In the case of humoral immunity, for example, ICOS signaling is critical for the differentiation of T follicular helper (Tfh) cells and development of germinal centers. Although C398.4A was generated against mouse ICOS, this antibody reportedly crossreacts with human, rhesus, and rat ICOS.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.
Development References (8)
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Araujo LM, Fert I, Jouhault Q, et al. Increased production of interleukin-17 over interleukin-10 by treg cells implicates inducible costimulator molecule in experimental spondyloarthritis. Arthritis Rheum. 2014; 66(9):2412-2422. (Clone-specific: Flow cytometry). View Reference
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Brenchley JM, Vinton C, Tabb B, et al. Differential infection patterns of CD4+ T cells and lymphoid tissue viral burden distinguish progressive and nonprogressive lentiviral infections.. Blood. 2012; 120(20):4172-81. (Clone-specific: Flow cytometry). View Reference
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Buonfiglio D, Bragardo M, Bonissoni S, et al. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol. 1999; 29(9):2863-2879. (Clone-specific: Flow cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay). View Reference
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Buonfiglio D, Bragardo M, Redoglia V, et al. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000; 30(12):3463-3467. (Clone-specific: Blocking, Flow cytometry). View Reference
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Chen Y, Shen S, Gorentla BK, Gao J, Zhong XP. Murine regulatory T cells contain hyperproliferative and death-prone subsets with differential ICOS expression. J Immunol. 2012; 188(4):1698-1707. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
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Klatt NR, Vinton CL, Lynch RM et al. SIV infection of rhesus macaques results in dysfunctional T- and B-cell responses to neo and recall Leishmania major vaccination. Blood. 2011; 118(22):5803-5812. (Clone-specific: Flow cytometry). View Reference
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Redoglia V, Dianzani U, Rojo JM, et al. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol. 1996; 26(11):2781-2789. (Immunogen: Bioassay, (Co)-stimulation, Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
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Xu H, Wang X, Lackner AA, Veazey RS. PD-1(HIGH) Follicular CD4 T Helper Cell Subsets Residing in Lymph Node Germinal Centers Correlate with B Cell Maturation and IgG Production in Rhesus Macaques. Front Biosci. 2014; 5(85):1-7. (Clone-specific: Flow cytometry). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.