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      Purified NA/LE Mouse Anti-Human CD51/CD61
      Product Details
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      BD Pharmingen™
      Vitronectin Receptor; Itgav/Itgb3; Integrins alpha V, beta 3
      Human (QC Testing), Chicken, Rabbit, Cow (Reported)
      Mouse BALB/c IgG1, κ
      Human Osteoclastomas
      Flow cytometry (Routinely Tested)
      1.0 mg/ml
      V S246, BP391
      AB_11153850
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      562165 Rev. 2
      Antibody Details
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      23C6

      The 23C6 monoclonal antibody specifically binds to the Integrin αvβ3 heterodimeric complex (CD51/CD61) that is expressed on osteoclasts, endothelial cells, macrophages, melanoma cells, some B cells and in very low amounts on platelets. This antibody has been reported to crossreact with chicken, bovine, and rabbit, but not with rat αvβ3. The integrin αvβ3 heterodimer, often referred to as the vitronectin receptor, binds several ligands in addition to vitronectin including, von Willebrand factor, thrombospondin, osteopontin, bone sialoprotein, neural adhesion molecule L1, laminin, fibronectin, and fibrinogen. The binding of some ligands to the vitronectin receptor has been reported to be inhibited by the 23C6 monoclonal antibody.

      562165 Rev. 2
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      562165 Rev.2
      Citations & References
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      Development References (9)

      1. Athanasou NA, Quinn J, Horton MA, McGee JO. New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Miner. 1990; 8(1):7-22. (Biology). View Reference
      2. Chuntharapai A, Bodary S, Horton M, Kim KJ. Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Exp Cell Res. 1993; 205(2):345-352. (Biology). View Reference
      3. Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M. The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989; 109(4):1817-1826. (Biology). View Reference
      4. Horton MA, Lewis D, McNulty K, Pringle JA, Chambers TJ. Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens. Cancer Res. 1985; 45(11):5663-5669. (Biology). View Reference
      5. Horton MA, Taylor ML, Arnett TR, Helfrich MH. Arg-Gly-Asp (RGD) peptides and the anti-vitronectin receptor antibody 23C6 inhibit dentine resorption and cell spreading by osteoclasts. Exp Cell Res. 1991; 195(2):368-375. (Biology). View Reference
      6. Nesbitt S, Nesbit A, Helfrich M, Horton M. Biochemical characterization of human osteoclast integrins. Osteoclasts express alpha v beta 3, alpha 2 beta 1, and alpha v beta 1 integrins. J Biol Chem. 1993; 268(22):16737-16745. (Biology). View Reference
      7. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      8. Usatyuk PV, Parinandi NL, Natarajan V.. J Biol Chem. 2006; 281(46):35554-35566. (Biology). View Reference
      9. Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
      View All (9) View Less
      562165 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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