Product Details
BD Pharmingen™
Itgam; Integrin αM chain; Mac-1 α chain; Integrin beta 2 alpha subunit
Rat (QC Testing)
Mouse BALB/c IgA, κ
Rat neutrophils
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Immunoprecipitation, Western blot (Reported)
0.5 mg/ml
AB_2129492
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Mouse IgA, κ Isotype Control RUO
Size
0.5 mg
Cat No.
553476
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
554980 Rev. 11
Antibody Details
WT.5
The WT.5 monoclonal antibody specifically binds to CD11b, the α subunit of Mac-1 found on neutrophils and myeloid cells, but not on lymphocytes. Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. Mac-1 mediates adhesion to ICAM-1 (CD54) and C3bi. Neutrophil binding to ICAM-1 is blocked by mAb WT.5.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
554980 Rev. 11
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554980 Rev.11
Citations & References
View product citations for antibody "554980" on CiteAb
Development References (2)
-
Tamatani T, Kitamura F, Kuida K, et al. Characterization of rat LECAM-1 (L-selectin) by the use of monoclonal antibodies and evidence for the presence of soluble LECAM-1 in rat sera. Eur J Immunol. 1993; 23(9):2181-2188. (Immunogen: Immunoprecipitation, Western blot). View Reference
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Watanabe T, Arakawa T, Fukuda T, Higuchi K, Kobayashi K. Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta. Am J Pathol. 1997; 150(3):971-979. (Clone-specific: Blocking). View Reference
554980 Rev. 11
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.