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Purified Mouse Anti-Human MCP-3
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
E. coli-expressed recombinant MCP-3
ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Intracellular staining (flow cytometry), Neutralization (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified 9H11 antibody (Cat. No. 551228) is useful as a capture antibody for a sandwich ELISA for measuring human MCP-3 protein levels. The purified 9H11 antibody can be paired with the biotinylated 6H5 antibody (Cat. No. 555034) as the detection antibody, with recombinant human MCP-3 (Cat. No. 554629) as the standard. Purified 9H11 antibody should be titrated to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human MCP-3 ranging from ~40 to 5,000 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section of our website or the chapter on ELISA in the Immune Function Handbook, both of which can be found at

Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MCP-4, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF-α, TNF-β, VEGF).

Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of plasma or serum samples.

Note 3: The  9H11 antibody is useful for immunofluorescent staining and flow cytometry. A PE conjugated format of the antibody is recommended for intracellular staining.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551228 Rev. 1
Antibody Details
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This antibody reacts with human monocyte chemotactic protein-3 (MCP-3). The immunogen used to produce the 9H11 hybridoma was E. coli-expressed recombinant MCP-3. This is a neutralizing antibody.

551228 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
551228 Rev.1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.