BD Pharmingen™ Purified Mouse Anti-Human CD44
Clone G44-26 (also known as C26) (RUO)
Flow cytometric analysis of CD44 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD44 (Cat. No. 555476, solid line histogram) or Purified Mouse IgG2b ĸ Isotype Control (Cat. No. 555740, dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988, both histograms). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Flow cytometric analysis of CD44 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD44 (Cat. No. 555476, solid line histogram) or Purified Mouse IgG2b ĸ Isotype Control (Cat. No. 555740, dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988, both histograms). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Product Details
BD Pharmingen™
Phagocytic glycoprotein 1; Pgp-1; H-CAM; Hermes; ECMR III; HUTCH-1
Human (QC Testing)
Mouse IgG2b, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
VI A092
AB_395868
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Purified Mouse IgG2b κ Isotype Control RUO
Size
0.1 mg
Cat No.
555740
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
555476 Rev. 6
Antibody Details
G44-26
The G44-26 monoclonal antibody specifically binds to the 80-95 kDa glycosylated type I transmembrane protein, CD44, also known as phagocytic glycoprotein-1 (Pgp-1). CD44 is the receptor for hyaluronic acid. CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. CD44 is also called extracellular matrix receptor type III and has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation. This antibody recognizes epitope 1 of CD44 antigen according to the HLDA workshop studies.
555476 Rev. 6
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555476 Rev.6
Citations & References
View product citations for antibody "555476" on CiteAb
Development References (5)
-
Galandrini R, Galluzzo E, Albi N, Grossi CE, Velardi A. Hyaluronate is costimulatory for human T cell effector functions and binds to CD44 on activated T cells. J Immunol. 1994; 153(1):21-31. (Biology). View Reference
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Günthert U. CD44: a multitude of isoforms with diverse functions. Curr Top Microbiol Immunol. 1993; 184:47-63. (Biology). View Reference
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Patel DD, Liao HX, Haynes BF. CD44 workshop panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:373-375.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Stamenkovic I, Amiot M, Pesando JM, Seed B. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Cell. 1989; 56(6):1057-1062. (Biology). View Reference
555476 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.