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      Purified Hamster Anti-Rat CD29
      Product Details
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      BD Pharmingen™
      Itgb1; Integrin β1 chain; Integrin beta-1; VLA-4 subunit beta
      Rat (QC Testing), Mouse (Tested in Development)
      Armenian Hamster IgM, κ
      Rat glomerular epithelial cells
      Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_395637
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Preliminary studies have shown that azide-containing antibody preparations non-specifically interfere with some in vitro adhesion assays. Therefore, we recommend the No Azide/Low Endotoxin (NA/LE™) format of the Ha2/5 antibody and NA/LE™ hamster IgM isotype control (Cat. No. 553957) for in vitro blocking studies. We recommend our immunohistochemistry formulation of purified anti-mouse CD29 mAb HMβ1-1, Cat. No. 550530, for immunohistochemical staining (IHC) of rat tissues. For IHC of mouse tissues, we recommend the use of purified anti-mouse CD29 mAb 9EG7 in our special formulation for immunohistochemistry, Cat. No. 550531.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      555003 Rev. 11
      Antibody Details
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      Ha2/5

      The Ha2/5 monoclonal antibody specifically binds to the 130 kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse thymocytes, splenocytes, and peripheral lymph node leukocytes. The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to collagen.

      555003 Rev. 11
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      555003 Rev.11
      Citations & References
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      Development References (2)

      1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen: Blocking, Immunoprecipitation). View Reference
      2. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
      555003 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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