BD Pharmingen™ PerCP-Cy™5.5 Mouse Anti-Human TGF-β1
Clone TW4-9E7 (RUO)
Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells (dashed line histogram) and human TGF-β1-transfected P3UI cells (solid line histogram) were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722) and washed with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PerCP-Cy™5.5 Mouse Anti-Human TGF-β1 antibody (Cat. No. 562423). The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of human TGF-β1 expressed by TGF-β1-transfected P3UI cells. Untransfected mouse P3UI myeloma cells (dashed line histogram) and human TGF-β1-transfected P3UI cells (solid line histogram) were fixed and permeabilized for 30 minutes with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722) and washed with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained with PerCP-Cy™5.5 Mouse Anti-Human TGF-β1 antibody (Cat. No. 562423). The flow cytometric fluorescence histograms were derived from gated events with the forward- and side light-scattering characteristics of intact cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Pharmingen™
TGFB1; TGFbeta1; TGF-beta-1; Transforming growth factor, beta 1; CED; LAP
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human TGF-β1 Transfected Cell Line
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_2737615
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
- PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation and Permeabilization Solution RUO
Size
125 mL
Cat No.
554722
Perm/Wash Buffer RUO
Size
100 mL
Cat No.
554723
562423 Rev. 1
Antibody Details
TW4-9E7
The TW4-9E7 monoclonal antibody specifically binds to Human Transforming Growth Factor beta-1 (TGF-β1). TGF-β1 is a potent multifunctional cytokine that positively or negatively regulates numerous processes including development, hematopoiesis, tissue remodeling, wound repair, innate and adaptive immunity as well as cancer and autoimmune diseases. TGF-β1 is formed by the enzymatic cleavage of the TGF-β1 propeptide that is encoded by the TGFB1 gene and comprised of the Latency Associated Peptide (LAP) and TGF-β1. Prior to secretion, the dimeric LAP-TGF-β1 propeptide is cleaved resulting in a biologically inactive form of dimeric TGF-β1 that is noncovalently associated with dimeric LAP (latent TGF-β1). This complex may be expressed on the surface of TGF-β1-producing cells or be further processed by proteolytic removal of LAP to release the biologically active mature form of the soluble TGF-β1 homodimer. Many different cell types synthesize TGF-β1 and express specific receptors for it. The TW4-9E7 antibody recognizes both the intracellular latent bound form of TGF-β1 along with the membrane bound form of TGF-β1.
562423 Rev. 1
Format Details
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
562423 Rev.1
Citations & References
View product citations for antibody "562423" on CiteAb
Development References (6)
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Derynck R, Akhurst RJ. Differentiation plasticity regulated by TGF-beta family proteins in development and disease. Nat Cell Biol. 2007; 9(9):1000-1004. (Biology). View Reference
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Derynck R, Jarrett JA, Chen EY, et al. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985; 316(6030):701-705. (Biology). View Reference
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Dünker N, Krieglstein K. Targeted mutations of transforming growth factor-beta genes reveal important roles in mouse development and adult homeostasis. Eur J Biochem. 2000; 267(24):6982-6988. (Biology). View Reference
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Miyazono K, Hellman U, Wernstedt C, Heldin CH. Latent high molecular weight complex of transforming growth factor beta 1. Purification from human platelets and structural characterization. J Biol Chem. 1988; 263(13):6407-6415. (Biology). View Reference
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Oida T, Weiner HL. Overexpression of TGF-β1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-β. J Immunol. 2010; 185(6):3529-3535. (Immunogen: Flow cytometry). View Reference
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Rubtsov YP, Rudensky AY. TGFbeta signalling in control of T-cell-mediated self-reactivity. Nat Rev Immunol. 2007; 7(6):443-453. (Biology). View Reference
562423 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.