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      PE Mouse Anti-Human Integrin α5 chain (CD49e)
      PE Mouse Anti-Human Integrin α5 chain (CD49e)
      Flow cytometric analysis of CD49e expression on human peripheral blood lymphocytes. Human whole blood was stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human Integrin α5 chain (CD49e) antibody (Cat. No. 555617; solid line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      PE Mouse Anti-Human Integrin α5 chain (CD49e)
      Flow cytometric analysis of CD49e expression on human peripheral blood lymphocytes. Human whole blood was stained with either PE Mouse IgG1 κ Isotype Control (Cat. No. 555749; dashed line histogram) or PE Mouse Anti-Human Integrin α5 chain (CD49e) antibody (Cat. No. 555617; solid line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
      Product Details
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      BD Pharmingen™
      Integrin α5; Integrin alpha-5; ITGA5; ITA5; FNRA; VLA-5; VLA5A
      Human (QC Testing)
      Mouse RBF/ DnJ IgG1, κ
      Human α5β1 Protein
      Flow cytometry (Routinely Tested)
      20 µl
      3678
      AB_395984
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555617 Rev. 7
      Antibody Details
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      IIA1

      The IIA1 monoclonal antibody specifically recognizes CD49e, a type I transmembrane glycoprotein which is also known as Integrin α5 Chain or VLA-5 α chain (VLA-5α).  Following translation, CD49e is cleaved into two disulfide-linked chains of 135 and 25 kDa that associate with β1 integrin (CD29) to form the VLA-5 (Integrin α5/β1) complex. The VLA-5 complex is a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, thymocytes, T cells, B cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone inhibits α5β1 binding of ligands. It is useful for studies of the expression and function of this integrin.

      555617 Rev. 7
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      555617 Rev.7
      Citations & References
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      View product citations for antibody "555617" on CiteAb

      Development References (3)

      1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
      2. Tran Van Nhieu G, Isberg RR. Bacterial internalization mediated by beta 1 chain integrins is determined by ligand affinity and receptor density. EMBO J. 1993; 12(5):1887-1895. (Biology). View Reference
      3. Tran Van Nhieu G, Isberg RR. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. J Biol Chem. 1991; 266(36):24367-24375. (Biology). View Reference
      555617 Rev. 7

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.