BD Pharmingen™ PE Mouse Anti-Human CD79b
Clone 3A2-2E7 (also known as SN8) (RUO)
Flow cytometric analysis of CD79b expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD79b (Cat. No. 557931) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color dot plot depicting CD79b expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.
Flow cytometric analysis of CD79b expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human CD79b (Cat. No. 557931) and FITC Mouse Anti-Human CD19 (Cat. No. 555412/560994). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The two-color dot plot depicting CD79b expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes.
Product Details
BD Pharmingen™
Igβ; B29; IGB; AGM6; CD79B
Human (QC Testing)
Mouse BALB/c IgG1, κ
Cell membranes from human B-prol ymphocytic leukemia (B-PLL) cells
Flow cytometry (Routinely Tested)
20 µl
V B037
AB_396949
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
PE Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555749
FITC Mouse Anti-Human CD19 RUO
Size
25 Tests
Cat No.
560994
557931 Rev. 4
Antibody Details
3A2-2E7
The 3A2-2E7 monoclonal antibody (also known as SN8) specifically recognizes CD79b. Immunoglobulin (Ig) antigen receptors are composed of a non-covalently-associated complex of Ig and two other proteins, Igα and Igβ, clustered as CD79a and CD79b, respectively. CD79b is a membrane glycoprotein of 229 residues, with a predicted relative molecular mass of 36-40 kDa. Its expression is restricted to B lineage cells. CD79b reportedly associates with surface IgM and is involved in signal transduction. The 3A2-2E7 antibody has similar reactivity characteristics as clone CB3-1. The 3A2-2E7 and CD3-1 antibodies specifically react with an epitope that is enhanced on certain B-cell leukemias such as prolymphocytic leukemia and lymphoma, but not on chronic lymphocytic leukemia.
557931 Rev. 4
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
557931 Rev.4
Citations & References
View product citations for antibody "557931" on CiteAb
Development References (4)
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Moreau EJ, Matutes E, A'Hern RP, et al. Improvement of the chronic lymphocytic leukemia scoring system with the monoclonal antibody SN8 (CD79b). Am J Clin Pathol. 1997; 108(4):378-382. (Biology). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Van Kooten C, Galibert L, Seon BK, Garrone P, Liu YJ, Banchereau J. Cross-linking of antigen receptor via Ig-beta (B29, CD79b) can induce both positive and negative signals in CD40-activated human B cells. Clin Exp Immunol. 1997; 110(3):509-515. (Biology). View Reference
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Zomas AP, Matutes E, Morilla R, Owusu-Ankomah K, Seon BK, Catovsky D. Expression of the immunoglobulin-associated protein B29 in B cell disorders with the monoclonal antibody SN8 (CD79b). Leukemia. 1996; 10(12):1966-1970. (Biology). View Reference
557931 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.