The 165903 monoclonal antibody specifically recognizes UL16-binding protein 2, 5, and 6 (ULBP-2/5/6). UL16 is a human cytomegalovirus (HCMV) glycoprotein that can bind to human ULBP proteins which share homology with MHC class I proteins. ULBP-2 is also known as RAET1H (retinoic acid early transcript), NKG2DL2 (NKG2D ligand 2), and ALCAN-alpha. ULBP-5 is likewise known as RAET1G whereas ULBP-6 is also referred to as RAET1L. The ULBP-2/5/6 proteins possess only the alpha 1 and alpha 2 Ig-like domains and have no capacity to bind peptides or interact with beta 2 microglobulin. All three proteins can be expressed on the cell surface as glycophosphatidylinositol (GPI)-linked forms whereas ULBP2 and ULBP5 also exist as transmembrane-anchored forms. These proteins may be expressed on various cells types during development or cells experiencing stress caused by high proliferative activity, infection, transformation, or exposure to mediators in inflammatory or autoimmune diseases. The ULBP-2/5/6 proteins can bind to NKG2D and thereby transduce activating signals into NK cells and costimulatory signals into activated CD8+ T cells resulting in enhanced cell-mediated cytotoxicity or cytokine production. The expression of ULBP-2/5/6 proteins on infected or tumor cells can play an important role in immune surveillance and homeostasis.
The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).