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BV786 Mouse Anti-Human ULBP-2/5/6
Product Details
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BD OptiBuild™
Human (Tested in Development)
Mouse IgG2a, κ
Human ULBP-2 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Cy is a trademark of GE Healthcare.
748134 Rev. 2
Antibody Details
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The 165903 monoclonal antibody specifically recognizes UL16-binding protein 2, 5, and 6 (ULBP-2/5/6). UL16 is a human cytomegalovirus (HCMV) glycoprotein that can bind to human ULBP proteins which share homology with MHC class I proteins. ULBP-2 is also known as RAET1H (retinoic acid early transcript), NKG2DL2 (NKG2D ligand 2), and ALCAN-alpha. ULBP-5 is likewise known as RAET1G whereas ULBP-6 is also referred to as RAET1L. The ULBP-2/5/6 proteins possess only the alpha 1 and alpha 2 Ig-like domains and have no capacity to bind peptides or interact with beta 2 microglobulin. All three proteins can be expressed on the cell surface as glycophosphatidylinositol (GPI)-linked forms whereas ULBP2 and ULBP5 also exist as transmembrane-anchored forms. These proteins may be expressed on various cells types during development or cells experiencing stress caused by high proliferative activity, infection, transformation, or exposure to mediators in inflammatory or autoimmune diseases. The ULBP-2/5/6 proteins can bind to NKG2D and thereby transduce activating signals into NK cells and costimulatory signals into activated CD8+ T cells resulting in enhanced cell-mediated cytotoxicity or cytokine production. The expression of ULBP-2/5/6 proteins on infected or tumor cells can play an important role in immune surveillance and homeostasis.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm.  BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

748134 Rev. 2
Format Details
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The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm.  BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter).  Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
Violet 405 nm
407 nm
786 nm
748134 Rev.2
Citations & References
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Development References (5)

  1. Cosman D, Müllberg J, Sutherland CL, et al. ULBPs, novel MHC class I-related molecules, bind to CMV glycoprotein UL16 and stimulate NK cytotoxicity through the NKG2D receptor.. Immunity. 2001; 14(2):123-33. (Biology). View Reference
  2. Lanier LL. NKG2D Receptor and Its Ligands in Host Defense.. Cancer Immunol Res. 2015; 3(6):575-82. (Biology). View Reference
  3. Raulet DH, Gasser S, Gowen BG, Deng W, Jung H. Regulation of ligands for the NKG2D activating receptor. Annu Rev Immunol. 2013; 31:413-441. (Biology). View Reference
  4. Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.. J Immunol. 2014; 193(4):1654-65. (Clone-specific: Flow cytometry). View Reference
  5. Wang R, Jaw JJ, Stutzman NC, Zou Z, Sun PD. Natural killer cell-produced IFN-γ and TNF-α induce target cell cytolysis through up-regulation of ICAM-1.. J Leukoc Biol. 2012; 91(2):299-309. (Biology). View Reference
View All (5) View Less
748134 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.