The TX24 monoclonal antibody specifically binds to the Poliovirus Receptor (PVR) which is also known as CD155, Nectin-like protein 5 (NECL5). PVR is a ~70 kDa nectin-like type I transmembrane glycoprotein that belongs to the PVR-related (PRR) family within the Ig superfamily. In addition to two cell surface PVR isoforms (alpha and delta), two secreted PVR isoforms (beta and gamma) have been reported that share the same three Ig domains but differ in their C-termini. PVR is expressed on monocytes, macrophages, endothelial cells, epithelia cells, CD34+ thymocytes, and neurons. In addition to serving as a receptor for poliovirus and cytomegalovirus, PVR functions as an adhesion molecule involved in cell-cell and cell-matrix adhesion through interaction with CD96 (TACTILE), Nectin 1-3 (CD111, CD112, CD113), CD226, and vitronectin. PVR promotes natural killer (NK) cell adhesion to and lysis of target cells.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.