The KJ1-26 monoclonal antibody specifically reacts with the DO-11.10 Clonotypic T-cell Receptor (TCR) of the BALB/c-derived DO-11.10 T-cell hybridoma and T lymphocytes from the DO-11.10 transgenic mouse (TgN[DO-11.10]10Loh). The DO-11.10 TCR, an 80-90-kDa (non-reduced) or 40-44-kDa (reduced) protein, is specific for the chicken OVA(323-339)/I-A[d] complex. The DO-11.10 T-cell hybridoma also responds strongly to chicken OVA/I-A[b] and jungle fowl OVA/I-A[d] and weakly to turkey OVA/I-A[d] and I-A[b]. The DO-11.10 mouse model is valuable for studies of T-cell immigration, immunoregulation, development, activation, and function. The KJ1-26 mAb was shown to block the antigen responses of the DO-11.10 T-cell hybridoma in vitro.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.