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This is a suggested alternate SKU for 560805.
Edited_562675_Image_1.png

Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were stained with FITC Hamster Anti-Mouse CD3 antibody (Cat. No. 553061/553062/561827) and BD Horizon™ BV421 Rat Anti-Mouse CD197 antibody (Cat. No. 562675). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CD197 for gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Edited_562675_Image_1.png

Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were stained with FITC Hamster Anti-Mouse CD3 antibody (Cat. No. 553061/553062/561827) and BD Horizon™ BV421 Rat Anti-Mouse CD197 antibody (Cat. No. 562675). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CD197 for gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Edited_562675_Image_1.png Edited_562675_Image_1.png Blue-Cap-Violet-Label.png

Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were stained with FITC Hamster Anti-Mouse CD3 antibody (Cat. No. 553061/553062/561827) and BD Horizon™ BV421 Rat Anti-Mouse CD197 antibody (Cat. No. 562675). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CD197 for gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Multicolor flow cytometric analysis of CD197 expression on BALB/c mouse thymocytes. Thymocytes were stained with FITC Hamster Anti-Mouse CD3 antibody (Cat. No. 553061/553062/561827) and BD Horizon™ BV421 Rat Anti-Mouse CD197 antibody (Cat. No. 562675). The two-color flow cytometric dot plot shows the correlated expression patterns of CD3 versus CD197 for gated events with the forward and side light-scatter characteristics of viable thymocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Horizon™
C-C chemokine receptor type 7; CD197; Ccr7; Cmkbr7; EBI1; Ebi1h
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a
Mouse CCR7 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2737716
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  7. Brilliant Violet™ 421 is a trademark of Sirigen.
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562675 Rev. 1
Antibody Details
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4B12

The monoclonal antibody 4B12/CCR7 reacts with the mouse C-C chemokine receptor type 7 (CCR7). CCR7 is also known as CD197 (previously known as EBI1, Ebi1h and CMKBR7) and plays a central role in mediating homeostatic B and T lymphocyte trafficking to and within secondary lymphoid tissues. CD197 is a seven-transmembrane, G-protein-coupled, 43 kDa glycoprotein receptor that is specific for the CC chemokines, MIP3ß/Exodus-3/ELC/CKb11/Scya19/CCL19 and 6Ckine/Exodus-2/SLC/TCA4/CKb9/Scya21/CCL21. The mouse Ccr7 gene is located on chromosome 11. CD197 (CCR7) is differentially expressed by subsets of thymocytes. Positive CD197 expression appears to be involved in the cortex-to-medulla migration of positively-selected thymocytes wherein they complete functional maturation including the establishment of central tolerance. It is most highly expressed by some mature medullary single-positive thymocytes. CD197 is also expressed by subsets of mature peripheral CD4+ and CD8+ T lymphocytes including naïve and regulatory T cells and central memory T cells. In addition, it is differentially expressed by subsets of B lymphocytes, dendritic cells, and Langerhans cells. CD197 serves as a homing receptor that helps guide these various cell types to and within lymphoid tissues. In this way, CCR7 supports protective immunity while safeguarding self tolerance. Reportedly, the 4B12/CCR7 antibody is not agonistic, is not blocked by CCL21 nor by physiologic levels of CCL19, nor does the antibody block the binding of CCL21 to CCR7. The immunogen used to generate the 4B12 hybridoma was a mouse CCR7-transfected rat cell line.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.

562675 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
562675 Rev.1
Citations & References
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View product citations for antibody "562675" on CiteAb

Development References (6)

  1. Britschgi MR, Link A, Lissandrin TK, Luther SA. Dynamic modulation of CCR7 expression and function on naive T lymphocytes in vivo. J Immunol. 2008; 181(11):7681-7688. (Clone-specific: Flow cytometry). View Reference
  2. Forster R, Davalos-Misslitz AC, Rot A. CCR7 and its ligands: balancing immunity and tolerance. Nat Rev Immunol. 2008; 8(5):362-371. (Biology). View Reference
  3. Kurobe, H., Liu, et al. CCR7-dependent cortex-to-medulla migration of positively selected thymocytes is essential for establishing central tolerance. Immunity. 2006; 24(2):165-177. (Biology). View Reference
  4. Ohl L, Mohaupt M, Czeloth N, et al. CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions. Immunity. 2004; 21(2):279-288. (Clone-specific: Flow cytometry). View Reference
  5. Ritter U, Wiede F, Mielenz D, Kiafard Z, Zwirner J, Korner H. Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.. J Leukoc Biol. 2004; 76(2):472-476. (Clone-specific: Flow cytometry). View Reference
  6. Schweickart VL, Raport CJ, Godiska R, et al. Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics. 1994; 23(3):643-650. (Biology). View Reference
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562675 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.