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BD Horizon™ BV421 Mouse Anti-Human CD45RO
Clone UCHL1 (RUO)
Multicolor flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.
Left Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody (Cat. No. 562641/562649; solid line histogram), or with a BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram).
Right Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).
Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot (CD45RO versus CD45RA expression) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.
Left Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody (Cat. No. 562641/562649; solid line histogram), or with a BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram).
Right Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).
Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot (CD45RO versus CD45RA expression) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.
Left Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody (Cat. No. 562641/562649; solid line histogram), or with a BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram).
Right Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).
Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot (CD45RO versus CD45RA expression) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.
Left Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody (Cat. No. 562641/562649; solid line histogram), or with a BD Horizon™ BV421 Mouse IgG2a, κ Isotype Control (Cat. No. 562439; dashed line histogram).
Right Panel: Whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD45RO antibody in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).
Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot (CD45RO versus CD45RA expression) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human IL-2-dependent T-cell line
Flow cytometry (Routinely Tested)
5 µl
IV N31
AB_2737696
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
BV421 Mouse IgG2a, k Isotype Control RUO
Size
50 µg
Cat No.
562439
PE Mouse Anti-Human CD45RA RUO
Size
100 Tests
Cat No.
555489
562649 Rev. 2
Antibody Details
UCHL1
The UCHL1 monoclonal antibody specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen). CD45RO is a type I transmembrane glycoprotein that has cytoplasmic protein tyrosine phosphatase activity and functions in signal transduction pathways. This CD45 isoform does not include amino acid sequences encoded by the variable CD45 exons A, B, or C. CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells. CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations. CD45RO binds to CD22.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
562649 Rev. 2
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
562649 Rev.2
Citations & References
View product citations for antibody "562649" on CiteAb
Development References (5)
-
Akbar AN, Terry L, Timms A, Beverley PC, Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988; 140(7):2171-2178. (Clone-specific: Flow cytometry). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986; 39(4):399-405. (Immunogen: Immunohistochemistry). View Reference
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Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986; 58(1):63-70. (Clone-specific: Flow cytometry, Immunohistochemistry, Immunoprecipitation). View Reference
-
Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
562649 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.