Preparation And Storage
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This antibody was developed for use in flow cytometry.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- BD Horizon Brilliant Ultraviolet 496 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
Companion Products
The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen. In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations. Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM. The HIS57 mAb does not stain granulocytes and thymocytes. Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles. This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.
Development References (3)
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Dammers PM, de Boer NK, Deenen GJ, Nieuwenhuis P, Kroese FG. The origin of marginal zone B cells in the rat. Eur J Immunol. 1999; 29(5):1522-1531. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
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Kroese FG, Butcher EC, Lalor PA, Stall AM, Herzenberg LA. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur J Immunol. 1990; 20(7):1527-1534. (Biology). View Reference
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Kroese FG, Wubbena AS, Opstelten D, et al. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur J Immunol. 1987; 17(7):921-928. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.