BD Pharmingen™ APC Mouse Anti-Human CD195
Clone 2D7/CCR5 (RUO)
Flow cytometric analysis of CD195 expression on human blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD195 (Cat. No. 556903/561748; solid line histogram) or APC Mouse IgG2a, κ Isotype Control (Cat. No. 555576; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms depicting CD195 (or Ig isotype control) expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Flow cytometric analysis of CD195 expression on human blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD195 (Cat. No. 556903/561748; solid line histogram) or APC Mouse IgG2a, κ Isotype Control (Cat. No. 555576; dashed line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms depicting CD195 (or Ig isotype control) expression were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Preparation And Storage
Recommended Assay Procedures
Immunophenotyping studies of chemokine receptors need to be performed on freshly collected whole blood (<24 hours). Incubation with the antibody should be done at room temperature in the dark. Cellular manipulation, such as Ficoll-Paque™ separation, freezing, or exposure to cold temperatures prior to staining have been shown to cause a decrease in staining intensity and inconsistent results.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Ficoll-Paque is a trademark of Amersham Biosciences Limited.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The 2D7/CCR5 monoclonal antibody specifically binds to the human chemokine receptor, CCR5. CCR5 is also known as CD195. CCR5 is a seven transmembrane-spanning G protein-associated molecule. CCR5 belongs to the beta chemokine receptor family. It is expressed on a subset of T lymphocytes (CD3+CD45RO+CD95+). CCR5 regulates lymphocyte chemotaxis activation and transendothelial migration during inflammation. It signals a response to at least three chemokines: RANTES and macrophage inflammatory protein-1 (MIP-1), and monocyte chemoattractant protein 2 (MCP-2). Additionally, CCR5 has been found to be a co-receptor for macrophage-tropic HIV-1 on CD4+ cells, a characteristic that is important in viral transmission. Reports indicate that individuals who have partial (heterozygous) or complete (homozygous) deletion of the CCR5 allele, demonstrate resistance to HIV infection. This antibody has been shown to block ligand and gp120 binding. It is also able to neutralize HIV infection.
Development References (8)
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Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996; 85(7):1135-1148. (Biology). View Reference
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Deng H, Liu R, Ellmeier W, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 1996; 381(6584):661-666. (Biology: Calcium Flux). View Reference
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Doranz BJ, Rucker J, Yi Y, et al. A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors. Cell. 1996; 85(7):1149-1158. (Biology). View Reference
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Dragic T, Litwin V, Allaway GP, et al. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 1996; 381(6584):667-673. (Biology). View Reference
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Hancock WW. Chemokines and the pathogenesis of T cell-dependent immune responses. Am J Pathol. 1996; 148(3):681-684. (Biology). View Reference
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Ponath P. Leukosite, Inc. . .
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Raport CJ, Gosling J, Schweickart VL, Gray PW, Charo IF. Molecular cloning and functional characterization of a novel human CC chemokine receptor (CCR5) for RANTES, MIP-1beta, and MIP-1alpha. J Biol Chem. 1996; 271(29):17161-17166. (Biology). View Reference
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Wu L, Paxton WA, Kassam N, et al. CCR5 levels and expression pattern correlate with infectability by macrophage-tropic HIV-1, in vitro. J Exp Med. 1997; 185(9):1681-1689. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.