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BD Pharmingen™ Purified Mouse Anti-Human PARP with Control
(RUO)Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6, Comp. No. 51-6639GR) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.
BD Pharmingen™ Purified Mouse Anti-Human PARP with Control
Purified Mouse Anti-Human PARP with Control
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20°C). Additional control lysate is sold separately.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Description | Quantity/Size | Part Number | EntrezGene ID |
---|---|---|---|
Purified Mouse Anti-Human PARP | 50 µg (3 ea) | 51-6639GR | N/A |
Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
Development References (2)
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Patel T, Gores GJ, Kaufmann SH. The role of proteases during apoptosis. FASEB J. 1996; 10(5):587-597. (Biology). View Reference
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Ranjit GB, Cheng MF, Mackay W, Whitacre CM, Berger JS, Berger NA. Poly(adenosine diphosphoribose) polymerase in peripheral blood leukocytes from normal donors and patients with malignancies. Clin Cancer Res. 1995; 1(2):223-234. (Immunogen: Dot Blot, Flow cytometry, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.