Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
United States (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PerCP Mouse Anti-Human CD61
      Product Details
      Down Arrow Up Arrow


      BD™
      Integrin β3; Integrin beta-3; GP3A; GPIIIa; ITGB3; ITB3
      Human
      Mouse BALB/c IgG1
      Purified platelet membrane glycoproteins
      Flow cytometry
      20 μL
      3690
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The monoclonal antibody is supplied as 12 µg purified immunoglobulin in 2.0 mL (6 µg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 25 µg in 2.0 mL (12.5 µg/mL). See vial label for PerCP concentration. PBS contains gelatin and 0.1% sodium azide.The vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

      Show More blue down arrow Show Less blue up arrow
      340506 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      RUU-PL7F12

      CD61, clone RUU-PL 7F12, is derived from hybridization of mouse P3X63.Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified platelet membrane glycoproteins.

      CD61 recognizes an Mr 110-kdalton protein, also known as gpIIIa, the common b subunit (integrin b3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR).

      340506 Rev. 1
      Format Details
      Down Arrow Up Arrow
      PerCP
      PerCP dye is part of the BD blue family of dyes. This dye is a fluorescent protein complex with an excitation maximum (Ex Max) of 481 nm and an emission maximum (Em Max) at 675 nm. PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405 nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PerCP
      Blue 488 nm
      481 nm
      675 nm
      340506 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "340506" on CiteAb

      Development References (13)

      1. Abrams CS, Ellison N, Budzynski AZ, Shattil SJ. Direct detection of activated platelets and platelet-derived microparticles in humans.. Blood. 1990; 75(1):128-38. (Biology). View Reference
      2. Fijnheer R, Modderman PW, Veldman H, et al. Detection of platelet activation with monoclonal antibodies and flow cytometry. Changes during platelet storage.. Transfusion. 1990; 30(1):20-5. (Biology). View Reference
      3. Fitzgerald LA, Steiner B, Rall SC, Lo SS, Phillips DR. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".. J Biol Chem. 1987; 262(9):3936-9. (Biology). View Reference
      4. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992; 69(1):11-25. (Biology). View Reference
      5. Jennings LK, Ashmun RA, Wang WC, Dockter ME. Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann's thrombasthenia in whole blood by flow cytometry.. Blood. 1986; 68(1):173-9. (Biology). View Reference
      6. Modderman PW. CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1017-1019.
      7. Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1025.
      8. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions.. Immunol Today. 1990; 11(7):225-7. (Biology). View Reference
      9. Shattil SJ, Hoxie JA, Cunningham M, Brass LF. Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation.. J Biol Chem. 1985; 260(20):11107-14. (Biology). View Reference
      10. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
      11. Wong DA, Springer TA. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:1664-1665.
      12. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
      13. von dem Borne AEGKr, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:997-999.
      View All (13) View Less
      340506 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.