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PerCP-Cy™5.5 Mouse Anti-Human CD33
Product Details
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BD™
Siglec-3; SIGLEC3; Sialic acid-binding Ig-like lectin 3; p67; gp67; My9
Human
Mouse BALB/c IgG1, κ
CD33-transfected FMY9S5 Cells
Flow cytometry
6.3 μg/mL
20 μL
IV M503
945
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The monoclonal antibody is supplied as 12 μg purified immunoglobulin in 2.0 mL (6 μg/mL) of phosphate-buffered saline (PBS). The FITC conjugate is supplied as 3 μg in 1.0 mL (3 μg/mL) of PBS. The PE conjugate is supplied as 24 μg in 2.0 mL (12 μg/mL) of PBS. The PerCP-Cy5.5 conjugate is supplied as 6.3 μg in 1.0 mL (6.3 μg/mL) of PBS. The PE-Cy7 conjugate is supplied as 12.5 μg in 0.5 mL (25 μg/mL) of PBS. The APC conjugate is supplied as 6.2 μg in 0.5 mL (12.4 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341650 Rev. 1
Antibody Details
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P67.6

The CD33 antibody, clone P67-6, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.

The CD33 antibody recognizes a human myelomonocytic antigen, with a molecular weight of 67 kilodaltons (kDa).

341650 Rev. 1
Format Details
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PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PerCP-Cy5.5
Blue 488 nm
482 nm
676 nm
341650 Rev.1
Citations & References
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View product citations for antibody "341650" on CiteAb

Development References (7)

  1. Andrews RG, Takahashi M, Segal GM, Powell JS, Bernstein ID, Singer JW. The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors. Blood. 1986; 68:1030-1035. (Biology).
  2. Andrews RG, Torok-Storb B, Bernstein ID. Myeloid-associated differentiation antigens on stem cells and their progeny identified by monoclonal antibodies.. Blood. 1983; 62(1):124-32. (Biology). View Reference
  3. Bernstein ID, Singer JW, Andrews RG, et al. Treatment of acute myeloid leukemia cells in vitro with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed.. J Clin Invest. 1987; 79(4):1153-9. (Biology). View Reference
  4. Dinndorf PA, Andrews RG, Benjamin D, Ridgway D, Wolff L, Bernstein ID. Expression of normal myeloid-associated antigens by acute leukemia cells.. Blood. 1986; 67(4):1048-53. (Biology). View Reference
  5. Foon KA, Todd RF. Immunologic classification of leukemia and lymphoma.. Blood. 1986; 68(1):1-31. (Biology). View Reference
  6. Köller U, Peschel CH. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:812-813.
  7. Terstappen LW, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukoc Biol. 1990; 48(2):138-148. (Biology). View Reference
View All (7) View Less
341650 Rev. 1

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Although not required, these products are manufactured in accordance with Good Manufacturing Practices.