Phosphate buffered saline with gelatin and 0.1% sodium azide.
Preparation And Storage
The PE conjugate is supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Anti-Hu–IL-13, clone JES10-5A2, is derived from the mouse myeloma P3X63Ag8 fused with splenocytes from Lewis rats immunized with recombinant human IL-13 expressed in mammalian cells.
Anti-Human Interleukin 13 (Anti-Hu–IL-13) recognizes a nonglycosylated protein of 132 amino acids with a molecular mass of 10 kilodaltons (kd).
340508 Rev. 1
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
Citations & References
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Burd PR, Thompson WC, Max EE. Activated mast cells produce interleukin 13. J Exp Med. 1995; 181:1373-1380. (Biology).
Dolganov G, Bort S, Lovett M, et al. Coexpression of the Interleukin-13 and Interleukin-4 genes correlates with their physical linkage in the cytokine gene cluster on human chromosome 5q23-31. Blood. 1996; 87:3316-3326. (Biology).
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Jung T, Wijdenes J, Neumann C, et al. Interleukin-13 is produced by activated human CD45RA+ and CD45RO+ T cells: Modulation by Interleukin-4 and Interleukin-12. Eur J Immunol. 1996; 26:571-577. (Biology).
Li H, Sim T, Alam R. IL-13 released by and localized in human basophils. J Immunol. 1996; 156:4833-4837. (Biology).
McKenzie ANJ, Culpepper JA, de Waal-Malefyt R, et al. Interleukin-13: A T-cell derived cytokine that regulates human monocyte and B-cell function. Proc Natl Acad Sci USA. 1993; 90:3735-3739. (Biology).
McKenzie ANJ, Zurawski G. Measurement of Interleukin-13. Current Protocols in Immunology. 1993; 90:3735-3739. (Biology).
Minty A, Chalon P, Derocq JM, et al. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature. 1993; 362:248-250. (Biology).
Muzio M, Re F, Sirone M, et al. Interleukin-13 induces the production of Interleukin-1 receptor antagonist (IL-1RA) and the expression of the mRNA for the intracellular (Keratinocyte) form of IL-1RA in human myelomonocytic cells. Blood. 1994; 83:1738-1743. (Biology).
Vannier E, de Waal Malefyt R, Salazar-Montes A, et al. Interleukin-13 (IL-13) induces IL-1 receptor antagonist gene expression and protein synthesis in peripheral blood mononuclear cells: Inhibition by an IL-4 mutant protein. Blood. 1996; 87:3307-3315. (Biology).
Zurawski G, deVries JE. Interleukin-13, an Interleukin-4–like cytokine that acts on monocytes and B-cells, but not on T-cells. Immunol Today. 1994; 15:19-26. (Biology).
deWall-Malefyt R, Figdor CG, Huijbens R, et al. Effects of IL-13 on phenotype, cytokine production, and cytotoxic function of human monocytes. J Immunol. 1993; 151:6370-6381. (Biology).