PE Mouse Anti-Human P-glycoprotein

BD™ PE Mouse Anti-Human P-glycoprotein

Name: PE Mouse Anti-Human P-glycoprotein
Brand: BD™
SKU: 340555

Clone 15D3

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: ABCB1; ABC20; CLCS; GP170; MDR1; P-GP; PGY1; pgp 170

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: MDR1-transfected BATV.2 cells

Application: Flow cytometry

Vol. Per Test: 20 μL

Entrez Gene ID: 5243

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The PE conjugate is supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340555 Rev. 1

Antibody Details

15D3

Anti–P-glycoprotein (P-gp), clone 15D3, is generated from the fusion of Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with a BA3T3 fibroblast line (BATV.2) transfected with the human MDR1 gene.

Anti–P-glycoprotein (P-gp) recognizes a 170-kdalton (kDa) protein from multidrug resistant (MDR) cells.

340555 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

340555 Rev.1

Citations & References

View product citations for antibody "340555" on CiteAb

Development References (10)

Akolkar PN, Gulwani-Akolkar B, Pergolizzi R, Bigler RD, Silver J. Influence of HLA genes on T cell receptor V segment frequencies and expression levels in peripheral blood lymphocytes. J Immunol. 1993; 150(7):2761-2773. (Biology). View Reference
Beck WT, Grogan TM, Willman CL, et al. Methods to detect P-glycoprotein–associated multidrug resistance in patients tumors: concensus recommendations. Cancer Res. 1996; 56:3010-3020. (Biology).
Chan HS, Haddad G, Zheng L, Bradley G, Dalton WS, Ling V. Sensitive immunofluorescence detection of the expression of P-glycoprotein in malignant cells.. Cytometry. 1997; 29(1):65-75. (Biology). View Reference
Dalton WS, Durie BG, Alberts DS, Gerlach JH, Cress AE. Characterization of a new drug-resistant human myeloma cell line that expresses P-glycoprotein.. Cancer Res. 1986; 46(10):5125-30. (Biology). View Reference
Drach D, Zhao S, Drach J, et al. Subpopulations of normal peripheral blood and bone marrow cells express a functional multidrug resistant phenotype.. Blood. 1992; 80(11):2729-34. (Biology). View Reference
Filipits M, Suchomel RW, Dekan G, et al. MRP and MDR1 gene expression in primary breast carcinomas.. Clin Cancer Res. 1996; 2(7):1231-7. (Biology). View Reference
Gupta S, Aggarwal S. P-glycoprotein in cells of the human immune system. The Immunologist 4/3. 1996; 86-90. (Biology).
Klimecki WT, Futscher BW, Grogan TM, Dalton WS. P-glycoprotein expression and function in circulating blood cells from normal volunteers.. Blood. 1994; 83(9):2451-8. (Biology). View Reference
List AF. Role of multidrug resistance and its pharmacological modulation in acute myeloid leukemia.. Leukemia. 1996; 10(6):937-42. (Biology). View Reference
Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). View Reference

View All (10)

340555 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.