Preparation And Storage
The FITC and PE conjugates are each supplied as 1 μg in 1.0 mL (1 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The Anti–bcl-2 antibody, clone 100, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the bcl-2α peptide (amino acids 41-54). The Anti–bcl-2 antibody recognizes a 26-kilodalton (kDa) protein encoded by the 230 kb bcl-2 protooncogene. The protein is a regulator of the apoptotic process, and elevated levels can provide resistance to cell death. The bcl-2 protein is expressed in the interior of cells, frequently localized to the mitochondrial membrane.
Development References (11)
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Hermine O, Haioun C, Lepage E, et al. Prognostic significance of bcl-2 protein expression in aggressive non-Hodgkin's lymphoma. Blood. 1996; 87:265-272. (Biology).
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Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). View Reference
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Reed JC. Double identity for proteins of the bcl-2 family. Nature. 1997; 387:773-776. (Biology).
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Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-5218. (Biology). View Reference
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Zamai L, Canonico B, Gritzapis A, et al. Intracellular detection of Bcl-2 and p53 proteins by flow cytometry: comparison of monoclonal antibodies and sample preparation protocols. J Biol Regul Homeost Agents. 2002; 16:289–302. (Biology).
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