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      Anti-Human Lambda FITC/Kappa PE/CD5 PerCP-Cy™5.5/CD10 PE-Cy™7/CD19 APC/CD20 APC-Cy™7

      BD Oncomark™ Anti-Human Lambda FITC/Kappa PE/CD5 PerCP-Cy™5.5/CD10 PE-Cy™7/CD19 APC/CD20 APC-Cy™7

      (RUO (GMP))
      Product Details
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      BD Oncomark™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with gelatin and 0.1% sodium azide.


      Description

      Anti-Lambda, clone 1-155-2, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with cells from BALB C/J mice immunized with human IgA1-λ myeloma protein.

      Anti-Kappa, clone TB28-2, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with cells from CB6 (BC57b x BALB/c) mice immunized with human IgG-κ myeloma protein.

      CD5, clone L17F12, is derived from hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells from BALB/c mice immunized with human T-acute lymphoblastic leukemia cells.

      CD10, clone HI10a, is derived the hybridization of mouse P3-63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with blasts from a patient with acute CALLA leukemia.

      CD19, clone SJ25C1, is derived from the hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with NALM1 + NALM16 cells.

      CD20, clone L27, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.

      Anti-Lambda is specific for lambda light chains of human immunoglobulins.

      Anti-Kappa is specific for kappa light chains of human immunoglobulins.

      CD5 recognizes a human T-lymphocyte antigen, Mr 67 kilodaltons (kd).

      CD10 (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen (CALLA), Mr 100 kd. The CD10 antigen is identical to human membrane–associated neutral endopeptidase (NEP; EC 3.3.24.11), also known as enkephalinase.

      CD19 (SJ25C1) recognizes a 90-kd antigen that is present on human B lymphocytes.

      The CD20 antigen is a phosphoprotein with a molecular weight of 35 or 37 kd, depending on the degree of phosphorylation. The antigen is not glycosylated.

      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      338427 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      Ig, λ Light Chain FITC 1-155-2 IgG1, λ 931
      Ig, κ light chain PE TB28-2 IgG1, κ 931
      CD5 PerCP-Cy5.5 L17F12 IgG2a, κ N/A
      CD10 PE Cy7 HI10a IgG1, κ 4311
      CD19 APC SJ25C1 IgG1, κ N/A
      CD20 APC-Cy7 L27 IgG1, κ 931
      338427 Rev. 1
      Citations & References
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      Development References (34)

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      16. Kubagawa H, Gathings WE, Levitt D, Kearney JF, Cooper MD. Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence.. J Clin Immunol. 1982; 2(4):264-9. (Biology). View Reference
      17. LeBien TW, McCormack RT. The common acute lymphoblastic leukemia antigen (CD10)--emancipation from a functional enigma.. Blood. 1989; 73(3):625-35. (Biology). View Reference
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      20. Letarte M, Vera S, Tran R, et al. Common acute lymphocytic leukemia antigen is identical to neutral endopeptidase. J Exp Med. 1988; 168(4):1247-1253. (Biology). View Reference
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      338427 Rev. 1

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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