PE Rat Anti-Mouse CD132

BD Pharmingen™ PE Rat Anti-Mouse CD132

Name: PE Rat Anti-Mouse CD132
Brand: BD Pharmingen™
SKU: 554457

Clone 4G3

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: Il2rg; IL-2Rγ; Commin gamma chain; Common γ; gamma c receptor; γc

Reactivity: Mouse (QC Testing)

Isotype: Rat LEW, also known as Lewis IgG2a, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_395404

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometric Analysis: This PE conjugated antibody is recommend for the immunofluorescent staining of γc receptor-bearing cells for flow cytometry.

Neutralization: The NA/LE™ format of the 4G3 antibody (Cat. No. 554454) strongly inhibits the IL-15-induced proliferative response of mouse CTLL cells but minimally inhibits the IL-2-induced responses of CTLL cells.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

PE Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 553930

554457 Rev. 1

Antibody Details

4G3

The 4G3 monoclonal antibody specifically binds to the common γ subunit (γc) shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptors also known as CD132. This receptor is expressed constitutively at low levels on most lymphocytes, myeloid cells, embryonic thymocytes and lymphoid cell lines, but not non-lymphoid cells. The γc receptor is a 75 - 80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated.

554457 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

554457 Rev.1

Citations & References

Development References (7)

Gillis S, Ferm MM, Ou W, Smith KA. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978; 120(6):2027-2032. (Clone-specific: Neutralization). View Reference
He YW, Adkins B, Furse RK, Malek TR. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor. J Immunol. 1995; 154(4):1596-1605. (Biology). View Reference
He YW, Malek TR. The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor. J Immunol. 1995; 155(1):9-12. (Biology). View Reference
Kondo M, Takeshita T, Higuchi M, et al. Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science. 1994; 263(5152):1453-1454. (Biology). View Reference
Kondo M, Takeshita T, Ishii N, et al. Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science. 1993; 262(5141):1874-1877. (Biology). View Reference
Malek TR, Furse RK, Fleming ML, Fadell AJ, He YW. Biochemical identity and characterization of the mouse interleukin-2 receptor beta and gamma c subunits. J Interferon Cytokine Res. 1995; 15(5):447-454. (Biology). View Reference
Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.

View All (7)

554457 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.