FITC Mouse Anti-Armenian and Syrian Hamster IgG

BD Pharmingen™ FITC Mouse Anti-Armenian and Syrian Hamster IgG

Name: FITC Mouse Anti-Armenian and Syrian Hamster IgG
Brand: BD Pharmingen™
SKU: 554026

Clone G192-1

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Armenian Hamster, Syrian Hamster (QC Testing), Mouse (Tested in Development)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Pooled Armenian and Syrian hamster IgG mAb

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395216

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

FITC Mouse Anti-Armenian and Syrian Hamster IgG may be used as a secondary reagent in immunofluorescent staining.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

554026 Rev. 9

Antibody Details

G192-1

Based on ELISA analysis, the G192-1 monoclonal antibody reacts specifically with Armenian hamster IgG2, IgG3, and IgG4, and Syrian hamster IgG2 monoclonal antibodies. The G192-1 mAb does not react with other hamster IgG groups or hamster IgM.

554026 Rev. 9

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

554026 Rev.9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.