Flow cytometric analysis of CD20 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD20 (Cat. No. 555621, solid line histogram) or Purified Mouse IgG2b κ Isotype Control (Cat. No. 555740, dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555899). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flouresence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
Flow cytometric analysis of CD20 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD20 (Cat. No. 555621, solid line histogram) or Purified Mouse IgG2b κ Isotype Control (Cat. No. 555740, dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555899). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Flouresence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
Product Details
BD Pharmingen™
MS4A1; B1; Bp35; LEU-16; S7
Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)
Mouse C57BL/6 IgG2b, κ
Human 6.16c1.3 B cell line
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
II B22; III B739, NL382; IV B201
AB_395987
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Purified Mouse IgG2b κ Isotype Control RUO
Size
0.1 mg
Cat No.
555740
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
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555621 Rev. 10
Antibody Details
2H7
The 2H7 monoclonal antibody specifically binds to CD20, encoded by the MS4A1 (Membrane-spanning 4-domains, subfamily A, member 1) gene. CD20 is a 33-37 kDa, unglycosylated four-transmembrane phosphoprotein. CD20 is expressed on pre-B-cells, resting and activated B cells, and follicular dendritic cells, but not plasma cells. Low level CD20 expression is observed on a small subset of normal circulating T lymphocytes. The CD20 molecule is involved in the regulation of B-cell activation.
555621 Rev. 10
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555621 Rev.10
Citations & References
View product citations for antibody "555621" on CiteAb
Development References (3)
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Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes. Cytometry. 1993; 14(2):193-204. (Biology). View Reference
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Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
555621 Rev. 10
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.