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Overview
BD® AbSeq Antibody-Oligos enable single-cell CITE-seq analyses (simultaneous detection of proteins and mRNA expression) and help deepen your understanding of protein biology to accelerate your single-cell research.
In addition to cell surface protein analyses, BD® AbSeq Antibody-Oligos can now also be used for intracellular CITE-seq.
Learn more from the BD® AbSeq Assay brochure
Check out our BD® AbSeq Immune Discovery Panel
Check out the Intracellular CITE-seq Assay using BD® AbSeq Antibody-Oligos
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/BD-AbSeq-Reagents-Overview.jpg)
FEATURES
The BD® AbSeq Assay provides enhanced cell type identification and high-dimensional protein profiling
The assay:
- Elucidates complex biological systems with distinct clustering of different cell subsets
- Enables interrogation of tens to hundreds of protein markers in a single experiment
- Integrates seamlessly with a catalog of RNA assays—whole transcriptome analyses, targeted RNA assays, TCR and BCR profiling and multiplexing—to serve as a single multiomics solution
- Provides a broad range of specificities including surface and intracellular proteins such as transcription factors, all part of a validated BD antibody portfolio
- Is designed for use with the BD Rhapsody™ Single-Cell Analysis Systems
- Provides customer-friendly analyses through integration with the BD Rhapsody™ Sequence Analysis Pipeline
APPLICATIONS
Detect up to 100 different cell surface markers using the BD® AbSeq Assay with high confidence
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/slide-1-images/Jurkat-Cell-Surface-Marker-Detection-Thumbnail.png)
![](https://www.bdbiosciences.com/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/slide-1-images/AbSeq-Assay-Simultaneous_Measurement-Thumbnail.png)
![](/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/slide-2-images/AbSeq-Assay-Mouse-Myeloid-HFD-Identification.png)
Single-cell AbSeq and targeted mRNA-Seq analyses of myeloid cell populations localized in adipose tissue from control and HFD mice. t-SNE visualization of differences in expression of Adgre1 (encodes F4/80) gene expression and CD11c protein expression (identified via AbSeq) in different cell clusters of myeloid cells from the adipose tissue of HFD mouse.
![](/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/AbSeq-Assay-Chronic-Lymphocytic-Leukemia-CD11c-CD5-Marker-Analysis.png)
Multiomic analysis of CLL samples and healthy donors. t-SNE clustering of healthy and CLL patient samples using mRNA or surface protein only or a combination of both. Expression of key cell surface markers identified by the BD® AbSeq Assay in both healthy and CLL samples.
![](/content/dam/bdb/products/reagents/single-cell-multiomics-reagents/abseq/slide-4-images/AbSeq-Conjugates-More-Defined-Peripheral-Blood-Mononuclear-Cell-Clustering-Full-width.jpg)
t-SNE clustering of PBMCs using mRNA or multiomic data. A, B. t-SNE coordinates are calculated for all PBMCs based on mRNA data only. Cells are colored based on cell type. C, D. Same coordinates as in A and B, but only T-cell subsets are displayed. Markers used for cell-type definition (protein data used for all markers except FcγRIIIa, for which mRNA data were used)—CD4 T-cells: CD3+CD4+; CD8 T-cells: CD3+CD8+; γδ T-cells: CD3+TCRγδ+; B cells: CD19+; Monocytes: CD14+; NK cells: CD3-CD45RA+ FcγRIIIA-high.
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Brochure
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Data Sheet
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A Comprehensive Characterization of Regulatory T Cells Using BD Rhapsody™ Single-Cell Analysis System
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Assessing Obesity-Induced Inflammation in Mice Through Single-Cell Multiomic Analysis
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Exploring Tumor Heterogeneity of Chronic Lymphocytic Leukemia Using Single-Cell Multiomics
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Simultaneous mRNA and Protein Quantification
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Presentations
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Protocols
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BD Rhapsody™ System Single-Cell Labeling with BD® AbSeq Ab-Oligos for Intracellular CITE-seq Protocol
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Flow Cytometry Analysis of BD® AbSeq Ab-Oligo and Sample Tag Expression Protocol
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mRNA Targeted and BD® AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit Protocol
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mRNA Targeted, Sample Tag, and BD® AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification and BD Single-Cell Multiplexing Kits Protocol
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Preparing Single-Cell Suspensions Protocol
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Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System Protocol
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Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD AbSeq Ab-Oligos (41 plex to 100 plex) 23-22951(01) Single-Cell Capture and cDNA Synthesis with the BD Rhapsody Single-Cell Analysis System
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Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD AbSeq Ab-Oligos (41 plex to 100 plex)
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Single-Cell Labeling with the BD® Single-Cell Multiplexing Kit and BD® AbSeq Ab-Oligos Protocol
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Single-Cell Labelling with BD® AbSeq Ab-Oligos Protocol
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Technical Note
For Research Use Only. Not for use in diagnostic or therapeutic procedures.