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Scientific Publications
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An Optimized 50-Color Flow Cytometry Panel for Analysis of T Cells and Dendritic Cells
Immune cell analysis at the periphery and at the tissue level has provided valuable insights into our understanding of how immune cells differentiate and what roles they play, as well as how they function in various diseases.
Using Single-Cell Multiomics to Identify a Novel Arthritis-Associated Osteoclast Precursor Macrophage
The authors of this article (Hasegawa, et.al, Nat.Immun 2019) have studied the formation of osteoclasts, which play a crucial role in arthritic bone erosion.
A Method to Study Tissue Microenvironments at Single-Cell Resolution
Spatial transcriptomics is a rapidly evolving field aiming to capture transcriptomic features and spatial relationships of cells within tissues.
Antigen-specific B cells and chronic infection: The OMIP panel
Originating in the bone marrow, antigen-specific B cells are critical in infection prevention and control through antibody production, professional antigen presentation and cytokine secretion.
NSCLC scRNA-seq Atlas shows variety and versatility of neutrophils in non-small cell lung cancer
Non-small cell lung cancer (NSCLC) contains a diversity of histological subtypes and specific mutational signatures. Single-cell RNA sequencing (scRNA-seq) technologies have enabled the characterisation of the NSCLC tumour microenvironment (TME).
A breakthrough OMIP panel on NK cells
OMIP-070, the first OMIP panel to profile NK cells from tumour tissue, proves to be an optimised flow cytometry immunophenotyping approach.
Targeted multiomics: visualise transcript and protein data together
Discrepancies between low-abundance transcripts and proteins make data visualisation challenging. Transcripts tend to be expressed at a much lower level than proteins.
Depolarised mitochondria in CD8+ TILs induce T-cell exhaustion
Mitochondria play a key role in sustaining the metabolic fitness of cells in response to metabolic perturbations by increased mitochondrial fusion and biogenesis. Mitochondria that are damaged during this process are cleared from the cell by mitophagy.
New phenotyping of human dendritic cells and monocyte cells
Recent technological developments in single-cell analysis have significantly advanced our understanding of the subset heterogeneity of antigen-presenting cells.
Using single-cell multiomics to study stem cell differentiation
In this study, they used single-cell analyses, transcriptional profiling and immunocytochemistry during long-term in vitro differentiation to assess the reliability of hCS derivation across multiple hiPSC lines and experiments.
Extricating Human Tumour Immune Alterations from Tissue Inflammation
This scientific paper summary presents how flow cytometry can differentiate between tumour immune alterations and non-malignant inflamed tissue.
A Standardized Flow Cytometry Network Study for the Assessment of Circulating Endothelial Cell Physiological Ranges
In this publication, a highly standardized protocol – based on LyotubeTM reagents and application settings – has been applied to a multicentre study for the evaluation of Circulating Endothelial Cells (CECs).
The Role of Immune Checkpoint Receptors in Cancer Immunity
The human immune system fights not only external pathogens but also clears internal abnormalities like dying and cancer cells.
A New 16-Color Flow Cytometry Panel Protocol for the Functional Phenotyping of Circulating Human Cytotoxic T Cells and NK Cells
Building a panel for the intracellular staining of immune cells could pose several challenges. A protocol published recently in Cell Press STAR Protocols takes these into account and provides a detailed workflow for efficiently phenotyping circulating human cytotoxic T cells and NK cells.
CLL Phenotyping with BD Rhapsody™ Targeted Panels and BD® AbSeq Assays
Exploring tumor heterogeneity of chronic lymphocytic leukemia using single cell multiomics